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Oral tumor vaccine and use thereof

A technology of tumor vaccines and oral vaccines, applied in the field of oral tumor vaccines, can solve the problems of ineffectiveness of tumor antigens, poor targeting, safety in use, lack of convenience and safety of oral vaccines, etc., achieving high safety and convenient use Effect

Active Publication Date: 2018-07-20
HUBEI SOUNDNY BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent provides an application of cell vesicles in the preparation of tumor vaccines. Cell vesicles derived from apoptotic tumor cells are used as antigens of tumor vaccines, which solves the problem that tumor vaccines cannot be effective against all tumor antigens, have poor targeting and Use of security issues
However, in this patent, the vaccination method of cell vesicles as a tumor vaccine is subcutaneous injection, which does not have the relative convenience and safety of an oral vaccine, and the immune prevention effect on abdominal tumors is not good.

Method used

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  • Oral tumor vaccine and use thereof
  • Oral tumor vaccine and use thereof
  • Oral tumor vaccine and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] [Example 1] Oral administration of tumor vesicles absorbed by ileal epithelial cells

[0047] 1. Experimental materials and reagents

[0048] H22 mouse hepatoma cells, fluorescent dyes: PKH26 (commercially available, with red fluorescence), E-cadherin antibody (commercially available, with green fluorescence), DAPI (nuclear dye, with blue fluorescence ), fluorescence microscope, and ultraviolet device are owned by the conventional cell ultra-clean workbench. The BABL / c mice used in the experiment were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., weighing about 18 grams.

[0049] 2. Experimental steps

[0050] 1) Cultivate H22 mouse liver cancer cells in DMEM cell culture medium to make the cell amount reach 2×10 7 / ml;

[0051] 2) After staining the above-mentioned H22 mouse liver cancer cells with PKH26 fluorescent dye, add fresh culture medium, then irradiate the above-mentioned H22 mouse liver cancer cells with ultraviolet light f...

Embodiment 2

[0057] [Example 2] Oral vaccines induce specific immune responses in mice

[0058] 1. Experimental materials and reagents

[0059] B16 mouse melanoma cells, fluorescently labeled antibodies against mouse CD8, CD4, CD3, and IFN-γ, C57 / BL6 mice used in the experiment were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., weighing about 18 grams.

[0060] 2. Experimental steps

[0061] 1) Obtain B16 mouse melanoma cell vesicles (B16-MP) according to the method described in Example 1;

[0062] 2) Divide 30 C57 / BL6 mice fed normally into 3 equal groups:

[0063] One group was used as an antacid combination experiment group, and the above prepared cell vesicles were used to immunize C57 / BL6 mice by oral perfusion, and the mice were pretreated with 0.05 mg omeprazole by intragastric administration 1 hour before each oral administration of tumor vaccines : specifically the first day of immunization for the first time (administration containing 5 × 10 7...

Embodiment 3

[0070] [Example 3] The effect of oral administration of tumor vesicles combined with antacids

[0071] 1. Experimental materials and reagents

[0072] The source of melanoma cells in B16 mice was the same as that in Example 2. The C57 / BL6 mice used in the experiment were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., weighing about 18 grams.

[0073] 2. Experimental steps

[0074] 1) Obtain the cell vesicle (B16-MP) of H22 mouse melanoma cells by the method of Example 1;

[0075] 2) Divide 30 C57 / BL6 mice fed normally into 3 equal groups:

[0076] One group was used as an antacid combination experiment group, and the above prepared cell vesicles were used to immunize C57 / BL6 mice by oral perfusion, and the mice were pretreated with 0.05 mg omeprazole by intragastric administration 1 hour before each oral administration of tumor vaccines : specifically the first day of immunization for the first time (administration containing 5 × 10 7 0.05ml...

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Abstract

The invention provides an application method of a cell vesicle in the preparation of an oral tumor vaccine. The oral tumor vaccine is a cell vesicle derived from an apoptotic tumor cell, an immunization subject firstly undergoes antacid pretreatment, and then the cell vesicle achieves immunoprophylaxis in an oral perfusion inoculation manner. The oral tumor vaccine can be extremely easily absorbedby the intestinal tract, causes strong immune response, and has a far better use effect than injected tumor vaccines.

Description

technical field [0001] The invention relates to an oral vaccine, in particular to an oral tumor vaccine. Background technique [0002] In recent years, as a disease that seriously threatens people's lives, tumor treatment has become the subject of research by many researchers. In addition to commonly known chemotherapy, surgical resection and other treatments, tumor vaccines are a new type of treatment. means has drawn increasing attention. [0003] The advantage of using cell vesicles as a tumor vaccine is huge. It is well known that cells are composed of cell membranes that wrap cell contents, and cell membranes are composed of phospholipid bilayers with protein molecules embedded in the middle. The centripetal pulling force formed by the filaments is maintained. When the cells are stimulated by external factors (such as ultraviolet rays, etc.) to undergo apoptosis, some protein filaments on the cytoskeleton attached to the cell membrane are broken or lose attachment, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K47/22A61P35/00A61P35/02
CPCA61K39/0011A61K47/22A61K2039/542A61K39/00A61P35/00A61P35/02
Inventor 黄波
Owner HUBEI SOUNDNY BIOLOGICAL TECH
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