Reaction curve generation method and device and optical detection system

Abstract

The invention relates to a reaction curve generation method. The reaction curve generation method comprises the following steps: 1) acquiring a transmission light signal value to obtain initial time sequence light quantity data and form an initial curve; 2) selecting a function model meeting the initial curve and fitting according to the initial curve to obtain a fitting curve; 3) comparing whether a determination coefficient of the fitting curve is no less than a target value or not; if so, entering step 5); otherwise, entering step 4); 4) calculating theoretical time sequence light quantitydata and determining repairing data; reforming an initial curve and returning back to step 2); 5) taking the fitting curve as a reaction curve. The reaction curve formed by the method disclosed by theinvention similarly restores a reaction process at maximum so that an absorbance value calculated by the reaction curve is more accurate. The invention also relates to a reaction curve generation device and an optical detection system.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a reaction curve generation method, device and optical detection system used in the medical or chemical industry. Background technique [0002] Taking the field of medical testing as an example, DD and FDP in blood coagulation items are usually detected by turbidimetry. [0003] The principle of the turbidimetric method is: the transmitted light generated by the light source and the optical fiber is emitted from one end to the transparent cuvette, and the other end of the cuvette uses a photocell to receive the transmitted light and convert it into a signal value. When the reaction continues, the turbidity of the material in the cuvette will increase, so as the reaction time prolongs, the intensity of the transmitted light and the converted signal value will gradually decrease. Through continuous detection for a period of time, a continuous decline can be obtained. By fitting ...

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Problems solved by technology

[0004] However, due to the influence of the reactant itself and other factors, agglomerated substances may be formed in the cuvette, and the agglomerated substances are easy to move randomly in the cuvette. When they block the path of the transmitted light, the collected reaction curve will appear downward. Concave V-shaped fluctuations, which easily lead to errors in the final calculation results; currently, for the V-shaped fluctuations, some fitting methods use the method of removing deviations greater than the threshold, but it is found through practice that this fitting method does not fit The deviation of the response curve is large

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