Applications of azelaic acid, adjuvants and vaccines containing azelaic acid

An azelaic acid and vaccine technology, applied in the field of immunology, can solve the problems of high price, serious side reactions, and insignificant immune response effects, and achieve the effects of low cost, small toxic and side effects, and enhancing humoral immune responses.

Active Publication Date: 2020-07-28
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of the existing vaccine adjuvants such as high price, serious side effects after injection, and insignificant effect of promoting immune response, the present invention provides the application of azelaic acid in the preparation of vaccine adjuvants, and provides an economical, effective, Safe and stable adjuvant and vaccine agent containing azelaic acid and preparation method thereof

Method used

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  • Applications of azelaic acid, adjuvants and vaccines containing azelaic acid
  • Applications of azelaic acid, adjuvants and vaccines containing azelaic acid
  • Applications of azelaic acid, adjuvants and vaccines containing azelaic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The hepatitis A vaccine containing azelaic acid adjuvant provided in this example is: the adjuvant obtained after dissolving 0.5 mg azelaic acid with 5 μ L of 75% ethanol, and every single HAV antigen (that is, the single injection used in animal experiments) is added to the adjuvant as usual. dose), and finally add physiological saline to 300 μL, mix evenly, and obtain hepatitis A vaccine containing azelaic acid adjuvant.

[0026] Wherein, azelaic acid is a commercially available product, and its molecular formula is C 9 h 16 o 4 , with a molecular weight of about 188.22, was purchased from adamas-beta in Switzerland; HAV antigen was a commercially available HAV antigen solution with a titer of 256EU / mL 18EU, which was purchased from the Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College.

[0027] Gained immunity test and the effect of the hepatitis A vaccine containing azelaic acid adjuvant are as follows:

[0028] A....

Embodiment 2

[0044] The Hepatitis A vaccine with azelaic acid adjuvant provided in this example is: after dissolving 1 mg azelaic acid in 10 μL of 75% ethanol, the adjuvant is routinely added to each single HAV antigen (that is, the single injection dose used in animal experiments) , and finally add physiological saline to 300 μL, mix evenly, and obtain hepatitis A vaccine containing azelaic acid adjuvant.

[0045] The immune test of the hepatitis A vaccine containing azelaic acid adjuvant obtained in this example is the same as in Example 1, and the results are shown in Table 2.

[0046] Table 2 shows the serum anti-HAV IgG antibody levels of mice in each experimental group within 16 weeks after using the adjuvant provided in Example 2.

[0047] Table 2

[0048]

[0049] It can be seen from the data analysis that except for the blank group, anti-HAVIgG was detected in all experimental groups after 4 weeks of immunization.

Embodiment 3

[0051] The Hepatitis A vaccine with azelaic acid adjuvant provided in this example is: Dissolve 2 mg of azelaic acid in 20 μL of 75% ethanol and then add the adjuvant to each single portion of HAV antigen (i.e. the single injection dose used in animal experiments) as usual , and finally add physiological saline to 300 μL, mix evenly, and obtain hepatitis A vaccine containing azelaic acid adjuvant.

[0052] The immune test of the hepatitis A vaccine containing azelaic acid adjuvant obtained in this example is the same as in Example 1, and the results are shown in Table 3.

[0053] Table 3 shows the serum anti-HAV IgG antibody levels of mice in each experimental group within 16 weeks after using the adjuvant provided in Example 3.

[0054] table 3

[0055]

[0056]

[0057] It can be seen from the data analysis that except for the blank control group, the mice in each group produced anti-HAV IgG antibodies 4 weeks, 8 weeks, 12 weeks, and 16 weeks after immunization. At t...

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Abstract

The invention relates to application of azelaic acid, an adjuvant containing azelaic acid and a vaccine agent. The dosage of azelaic acid as an adjuvant in a vaccine is that single vaccine liquid contains 0.5 to 3mg of azelaic acid, and the adjuvant containing the azelaic acid is prepared from 5 to 30 microliters of ethanol with concentration of 75v / v percent and 0.5 to 3mg of azelaic acid. The adjuvant disclosed by the invention has the advantages of easily-available raw materials, simple vaccine preparation process, low cost, stable performance and little toxic and side effects; moreover, the adjuvant is used safely and reliably in a range of immunizing dose, humoral immune response of antigenic specificity can be effectively induced, and the humoral immune response of the antigenic specificity can be obviously enhanced, so that an effect is superior to an adjuvant-free group and a single adjuvant group, and the effect is equivalent to an aluminum adjuvant.

Description

technical field [0001] The invention relates to the application of azelaic acid, an adjuvant containing azelaic acid and a vaccine agent, belonging to the field of immunology. Background technique [0002] Vaccine adjuvant is a substance that can enhance the body's immune response to the antigen or change the immune response when it is injected with the antigen or pre-injected into the body, but it has no antigenicity itself. A comprehensive and durable immune response can be induced in immunocompromised populations such as the elderly, children, and immunocompromised persons. Since its discovery in the early 20th century, adjuvants have been used to improve and enhance the immune response of vaccines to the body, such as aluminum adjuvants. However, aluminum adjuvants are not suitable for recombinant protein and subunit vaccines, and can also cause adverse reactions, such as inflammation, stimulate local erythema, granuloma, subcutaneous nodules, contact dermatitis, IgE-me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61P37/04A61K39/205A61K39/29A61P31/14A61P31/20
CPCA61K39/12A61K39/39A61K2039/55511A61P31/14A61P31/20A61P37/04C12N2730/10134C12N2760/20134C12N2770/32434
Inventor 胡云章李晓红王海漩胡凝珠乌美妮孙静李彦涵李建芳施建东
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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