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Mutant gene Oslrt1 for controlling rice non-lateral-root trait, detection and application thereof

A technique for mutating genes and lateral roots, applied in its detection and application, and controlling the field of mutant gene Oslrt1 without lateral roots in rice, which can solve the problems of unknown biological functions and few

Active Publication Date: 2018-07-31
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In 2006, jain identified 31 family members of Aux / IAA in rice using bioinformatics methods, but the biological functions of specific members are not known much

Method used

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  • Mutant gene Oslrt1 for controlling rice non-lateral-root trait, detection and application thereof
  • Mutant gene Oslrt1 for controlling rice non-lateral-root trait, detection and application thereof
  • Mutant gene Oslrt1 for controlling rice non-lateral-root trait, detection and application thereof

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with specific examples.

[0023] Embodiment The localization process of the mutant gene Oslrt1 controlling rice without lateral root traits in the present invention

[0024] 1. Obtaining Mutants

[0025] Chemical mutagen sodium azide (10 -3 mol / L, NaN3) to treat wild-type rice seeds ‘Daily’ for 6 hours, and obtain M2 seeds after seedling cultivation, and M2 seeds were sown in 2,4-D aqueous solution (10 -6 mol / L, pH 5.0), screen the 2,-D resistant mutant materials whose roots are different from those of the wild type, obtain M3 seeds, continue planting, and carry out 2,4-D resistance identification until a stable mutant without lateral roots is obtained Material RM109, such as figure 1 , Reference Hao Zaibin (Journal of Nuclear Agriculture, 2002, 16(1): 15-19).

[0026] 2. Gene mapping

[0027] The obtained no lateral root mutant RM109 was crossed with Miryang 23 and then selfed to construct a segr...

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Abstract

The invention discloses mutant gene Oslrt1 for controlling rice non-lateral-root trait, detection and application thereof, and the genomic full length of the gene is 2672 bp, contains 5 exons, and hasa G-A base mutation at the 15th position of a second exon. The nucleotide sequence is as shown in SEQ ID NO.1, the mutant gene hinders the development of lateral roots of rice, and a mutant has almost no lateral root growth. The mutant gene Oslrt1 can be used to regulate the expression of a protein for encoding the rice non-lateral-root trait in rice breeding, and rice with excellent roots can bebred.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to a mutant gene Oslrt1 for controlling the trait of no lateral root in rice, its detection and application. Background technique [0002] Lateral roots are an important part of the plant root system and an important organ for plants to absorb water and nutrients from the outside world. Rice is an important food crop, and it is of great significance to study the occurrence of lateral roots. [0003] Studies have found that some members of the Aux / IAA family play a very important role in the development of lateral roots. Generally speaking, there are relatively few studies on the Aux / IAA family in rice. The completion of the fine map of the rice genome in 2005 has accelerated the development of rice. Research. [0004] In 2006, jain identified 31 family members of Aux / IAA in rice using bioinformatics methods, but the biological functions of specific members are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/6895C12N15/11
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨瑞芳白建江朴钟泽方军
Owner SHANGHAI ACAD OF AGRI SCI