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Annular vertical illumination device for large-field high-resolution fluorescence microscope

A technology for fluorescent microscopes and lighting devices, applied in microscopes, optics, optical components, etc., can solve the problems of complex switching process and low operational flexibility, and achieve the effects of simplifying the overall system, improving utilization rate, and reducing chromatic aberration

Pending Publication Date: 2018-07-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above contrast technologies all need to add phase contrast push-pull plate, dark field push-pull plate and other structures on the aperture stop surface, the switching process is complicated and the operation flexibility is low.

Method used

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  • Annular vertical illumination device for large-field high-resolution fluorescence microscope
  • Annular vertical illumination device for large-field high-resolution fluorescence microscope
  • Annular vertical illumination device for large-field high-resolution fluorescence microscope

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Embodiment

[0022] like figure 1 As shown, the lighting device of the present invention includes a high-power laser light source 1 , an axicon lens 2 , an annular condenser lens 3 , a reflection ring 4 , and a sample 5 .

[0023] Step 1: The collimated monochromatic light (fluorescent excitation light) emitted by the high-power laser light source 1 is transformed into a ring beam through the axicon lens 2. The power of the high-power laser light source 1 is at least 200mW. The principle of the axicon lens 2 is as follows figure 2 As shown, when refracting light, follow Snell's law:

[0024] nsin(α)=sin(α+β)

[0025] Where n is the refractive index of the glass, α is the cone angle of the axicon, and β is the deflection angle generated by the refracted light and the optical axis.

[0026] The axicon is a lens with a conical surface and a plane. Its flat side faces the collimation and can convert the collimated circular beam into a ring beam. The material of the axicon 2 used in this dev...

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Abstract

The invention discloses an annular vertical illumination device for a large-field high-resolution fluorescence microscope. The annular vertical illumination device comprises a high-power laser light source, a taper lens, an annular focusing lens, a reflection ring and a sample, wherein collimating monochromatic light emitted by the high-power laser light source is converted into an annular light beam through the taper lens, and the annular light beam is focused through the annular focusing lens, and is then reflected to the surface of the sample through the reflection ring. Compared with the traditional vertical illumination device of the large-field high-resolution fluorescence microscope, the illumination light is directly subjected to oblique incidence to the surface of the tested sample in the form of the larger-radius annular light from the outer side of an objective lens cone without going through an objective lens, so that the use ratio of the off-axis illumination light beam iseffectively improved; an illumination light path and an imaging light path are separated without a dichroscope placed at 45 degrees, the overall system is simplified, the chromatic aberration of thesystem is reduced; and the annular vertical illumination device provides the imaging with the much evener energy, and is particularly suitable for being matched with the fluorescence microscopy.

Description

technical field [0001] The invention mainly relates to the field of illumination of microscopes, in particular to an annular epi-illumination device for a large-field-of-view high-resolution fluorescence microscope. Background technique [0002] After a long period of development, the microscope has a relatively complete illumination system, including a light source, a collector lens, a field diaphragm, a condenser lens, an aperture diaphragm, and an auxiliary lens. There are two main types of microscope illumination, transmissive illumination and epi-illumination (reflective) illumination. These two lighting methods are selected according to different observation objects: transillumination is mainly used for transparent specimens, such as transparent biological cell slices; The reflected light is imaged by the objective lens. Microscope illumination can be divided into central illumination and oblique illumination. The central axis of the illumination source for central i...

Claims

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Application Information

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IPC IPC(8): G02B21/00G02B21/06
CPCG02B21/0032G02B21/0076G02B21/06
Inventor 沈亦兵胡静陈浩
Owner ZHEJIANG UNIV