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Rapid measurement method for content of homocysteine in dried blood spot

A technology of homocysteine ​​and a detection method, which is applied in the field of rapid detection of homocysteine ​​content, can solve the problems of troublesome derivatization and biochemical steps, not suitable for rapid detection of homocysteine ​​Hcy, expensive reagents, etc.

Active Publication Date: 2018-08-03
HANGZHOU BAICHEN MEDICAL LAB CO LTD +1
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Problems solved by technology

For example, taking the HPLC method as an example, when using HPLC technology to detect the content of homocysteine ​​Hcy, it is necessary to perform sample pretreatment for homocysteine ​​Hcy, and derivatization is required for the pretreatment of homocysteine ​​Hcy Steps, derivatization and biochemical steps are cumbersome to deal with, and cannot be applied to the rapid detection of homocysteine ​​Hcy content
In addition, the specificity of biochemical and immunological methods is not ideal, and there is a problem that the reagents are relatively expensive
[0005] All in all, there is no universally recognized detection method for homocysteine ​​Hcy in the existing clinic

Method used

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  • Rapid measurement method for content of homocysteine in dried blood spot
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  • Rapid measurement method for content of homocysteine in dried blood spot

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Embodiment Construction

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present invention belong to the protection scope of the present invention.

[0048] Those skilled in the art should understand that in the disclosure of the present invention, the terms "vertical", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present invention and The above terms should not be const...

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Abstract

The invention discloses a rapid measurement method for content of homocysteine in a dried blood spot and applies to content measurement of homocysteine in a dried blood spot sample. The method comprises the following steps: obtaining a quantitative standard correction curve of homocysteine; preparing a sample for measurement; performing high-throughput liquid chromatographic separation on homocysteine; detecting homocysteine with tandem mass spectrometry; obtaining content of homocysteine. The method has the advantages of short measurement time, high throughput, high detection sensitivity, high specificity and relatively simple pretreatment process, and has a significance in clinical disease diagnosis and screening.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a rapid detection method for homocysteine ​​content in dried blood slices, wherein the rapid detection method for homocysteine ​​content in dried blood slices is applicable to high-throughput liquid chromatography tandem mass spectrometry Fast and efficient detection of homocysteine ​​in dried blood films. Background technique [0002] Homocysteinemia (Hyperhomocysteinemia, Hcy) is a sulfur-containing non-essential amino acid and an important intermediate product in the metabolic cycle of methionine. The main metabolic destination of homocysteine ​​Hcy is as follows: ① Released into the extracellular fluid ②Under the action of methionine synthase, N5-methyltetrahydrofolate is used as the methyl donor and vitamin B12 is used as the coenzyme factor to finally generate tetrahydrofolate and methionine ③The participation of serine in cystathionine β synthase Next, cond...

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 吴超超胡志飞刘鹏刘纪松江思高强彭军
Owner HANGZHOU BAICHEN MEDICAL LAB CO LTD
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