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Assay for determining antibiotics in waste

A technology of antibiotics and microorganisms, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems that affect the normal function of the microorganisms, the results are difficult to interpret, and the results are unreliable.

Inactive Publication Date: 2018-08-03
CENTRIENT PHARMA NETHERLANDS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Unfortunately, when applying such assays to substrates that are more complex than milk, e.g. containing many other components or even (semi)solid wastes such as filter cakes, mycelia or concentrated residues from fermentation processes ), the results are difficult to interpret and therefore unreliable
If the indicator change is a change in the color of the indicator molecule, the change is often difficult to observe or interpret
Another possible disadvantage is that other non-antimicrobial components in the composite sample interfere with the normal function of the assayed microorganisms

Method used

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  • Assay for determining antibiotics in waste
  • Assay for determining antibiotics in waste
  • Assay for determining antibiotics in waste

Examples

Experimental program
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Effect test

example 1

[0069] Manual for Determining Antibiotics in Wastewater

[0070] The following are non-limiting examples of what may be included as a manual in a kit of the invention.

[0071] environment:

[0072] Ensure that the environment in which the antibiotic assay is performed is free of β-lactams. If this cannot be guaranteed in the room itself, a clean bench can be used as an alternative.

[0073] Equipment Accessories:

[0074] - Centrifuge, 2ml centrifuge tube (rotating speed 15.000rpm)

[0075] - Water bath whose temperature can be set between 37°C and 65°C

[0076] -pH meter

[0077] - Freezer with separate compartment for storing samples; closed box inside the freezer can be used as an alternative

[0078]- Eppendorf pipettes for 10μl-100μl and 100μl-1000μl

[0079] - Eppendorf pipette points for 100 μl box 1000 μl

[0080] -2.0ml centrifuge tube

[0081] - 10ml centrifuge tube with screw cap

[0082] - 50ml Greiner centrifuge tube with screw cap

[0083] -Milli ...

example 2

[0154] Determination of antibiotics in samples with and without milk fortification

[0155] Two samples, blank (water) and 2 ppb penicillin were analyzed using a commercially available (DSM Food Specialties B.V., Delft, Netherlands) Delvotest SP NT (similar to the supplier's manual) GK test solution. Fill the two ampoules with blank samples. Milk was added to one (in this example plain low fat milk from a Dutch supermarket) and the same amount of water was added to the other according to the instructions given in Example 1. Additionally, two ampoules were filled with 2 ppb penicillin G K test solution and again following the instructions described in Example 1, milk was added to one and the same amount of water was added to the other. The ampoules filled with samples were incubated at 64°C + / - 2°C and the color of the agar in the ampoules was recorded visually at several time points. The experiments described above were performed in five replicates and the average observati...

example 3

[0159] Determination of antibiotics in samples with and without milk fortification

[0160] Example 2 was repeated with various other samples, such as in-process waste streams and extracts of fermented mycelium filter cakes, and in the absence of antibiotics in the samples, the effect observed in Example 2 was confirmed (compared to the absence of The color of the indicator in the agar layer of the Delvotest SP NT test ampoule in the presence of milk changes faster compared to that of milk).

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PUM

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Abstract

The present invention relates to a simple and easy-to-use method for the rapid determination of the presence of an antibiotic in a waste such as e.g. liquid or solid waste streams from plants. The present invention also relates to a kit comprising an assay and a manual for the rapid determination of the presence of an antibiotic in a waste.

Description

technical field [0001] The present invention relates to a simple and easy-to-use method for rapidly determining the presence of antibiotics in waste, such as liquid or solid waste streams from factories. The invention also relates to a kit comprising an assay and a manual for the rapid determination of the presence of antibiotics in waste. Background technique [0002] Antimicrobial resistance is one of the globally important issues that is no longer a prediction of the future, but is happening all over the world and has the potential to affect anyone at any age in any country. Antimicrobial resistance - when bacteria change so that antibiotics no longer work on people who need them to treat infections - is now a major threat to public health. There is a growing public call for global collective action to address this threat, including proposals for an international treaty on antimicrobial resistance. Antibiotic resistance around the world is not well mapped, but the count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18C12Q1/00
Inventor 伦德特·马里纳斯·哈内马埃杰尔狄瑞扎·钱德勒·加戈萨
Owner CENTRIENT PHARMA NETHERLANDS BV