Method for extracting saponin and coproducing kaempferol from tea-oil tea cakes

A technology of camellia oleifera and kaempferol, which is applied in the direction of steroids and organic chemistry, can solve the problems of waste of resources, etc., and achieve the effects of low production cost, cost reduction and long service life

Active Publication Date: 2018-08-10
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to technical limitations, camellia oleifera has not been well developed and utilized, resulting in a great waste of resources

Method used

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  • Method for extracting saponin and coproducing kaempferol from tea-oil tea cakes

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Such as figure 1 As shown, the raw material of camellia oleifera was crushed and passed through a 80-mesh sieve; 1 kg of camellia oleifera powder was weighed, added 10L of 60% ethanol, extracted at 60°C for 4 h, filtered, and the filtrate was collected; at an operating pressure of 0.15 Mpa, , filter the filtrate through a ceramic membrane with a pore size of 0.05 μm, and concentrate the permeate to 4 L by hydraulic pressure; add 0.8 L of 8 mol / L HCl to hydrolyze for 3 h, collect the solid phase, wash with cold water, and dry in vacuum to obtain kaempferol 5.6 g, the purity is 90.7%; Concentrate the liquid phase, add 8 mol / L NaOH to adjust the pH=5, use AB-8 macroporous resin to absorb, wash with water until the effluent is colorless, wash with 0.2‰ NaOH until the outflow The solution was colorless, then washed with water until the effluent was neutral, and finally eluted with 80% ethanol, collected the ethanol eluate, concentrated under reduced pressure, and freeze-dri...

Embodiment 2

[0028] Such as figure 1 As shown, the raw material of camellia oleifera was crushed and passed through a 50-mesh sieve; 10 kg of camellia oleifera powder was weighed, 120 L of 80% ethanol was added, extracted at 70°C for 6 h, filtered, and the filtrate was collected; the operating pressure was 0.1 Mpa , the filtrate is filtered through a ceramic membrane with a pore size of 0.1 μm, and the permeate is hydraulically concentrated until it has no alcohol smell; the concentrate is diluted with water to the volume of the original permeate, and 1 L of 1 % β -Glucosidase, hydrolyzed at 50°C for 24 h, collected the solid phase, washed with cold water, and dried in vacuum to obtain 62.8 g of kaempferol with a purity of 92.1%; the liquid phase was concentrated, adsorbed with D3520 macroporous resin, and then washed with Wash with water until the effluent is colorless, wash with 0.3‰ NaOH until the effluent is colorless, then wash with water until the effluent is neutral, and finally el...

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Abstract

The invention discloses a method for extracting saponin and coproducing kaempferol from tea-oil tea cakes. The method comprises the following specific steps: taking the tea-oil tea cakes as raw materials; extracting with an ethanol solution, filtering through a ceramic membrane and concentrating a permeation solution; hydrolyzing a permeation concentration solution to obtain a solid phase, i.e., the kaempferol; concentrating a liquid phase, adsorbing through macroporous resin, resolving and drying to prepare the saponin. According to the method disclosed by the invention, the tea-oil tea cakesare used as the raw materials; the raw materials are sufficient and the cost is low; a technological route is simple to operate and has moderate conditions and the purity of a product is high; a solution is provided for comprehensive utilization of tea-oil tea resources and improvement of economic value.

Description

technical field [0001] The invention relates to a method for extracting saponin from camellia oleifera and co-producing kaempferol, and belongs to the technical field of extracting bioactive components from camellia oleifera. Background technique [0002] Saponin ( Tea Saponin ) is a pentacyclic triterpenoid saponin, which is mainly composed of three parts: ligand, sugar body and organic acid. Studies have shown that saponin has strong hydrophilic groups and lipophilic groups, which can effectively reduce the surface tension of aqueous solutions. It is a typical non-ionic natural surfactant with emulsifying, moisturizing, decontamination, dispersion, hair Foaming, foam stabilizing and other surface activities; at the same time, it has anti-oxidation, hemolysis and fish poisoning, insecticide and mollusc killing, anti-seepage and anti-inflammation, anti-bacterial, anti-mutation, anti-hypertension, stimulating biological growth, inhibiting alcohol absorption and protecting th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07D311/30C07D311/40
CPCC07D311/30C07D311/40C07J63/008
Inventor 覃江克唐文迪黎国庆丁耀洋焦兵
Owner GUANGXI NORMAL UNIV
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