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A Multiple PCR Method for Identifying the Purity of Melon Seeds

A hybrid seed purity and seed technology, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of multiple PCR melon seed purity identification that have not yet been seen, and achieve shortened working hours, high accuracy, and reduced The effect of consumption

Active Publication Date: 2021-10-19
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But generally speaking, in the prior art, there is no good related report on the identification of the purity of melon seeds by using multiplex PCR technology.

Method used

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  • A Multiple PCR Method for Identifying the Purity of Melon Seeds
  • A Multiple PCR Method for Identifying the Purity of Melon Seeds
  • A Multiple PCR Method for Identifying the Purity of Melon Seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0046] (1) Extraction and preparation of DNA templates from materials to be tested

[0047] Generally speaking, when extracting muskmelon genomic DNA, it is convenient and easy to operate with young leaves at the seedling stage. The specific process is briefly introduced as follows.

[0048] The melon seeds to be tested were soaked in warm soup to accelerate germination. Sow 10 plants of DHL92 and TopMarker materials in seedling trays respectively as control group 1 and control group 2. The fresh-keeping box was used as the experimental group. After sowing, three layers of absorbent paper were covered on the top of the seeds and watered, and the seedlings were raised in the light incubator, and the DNA of the young leaves was extracted when about two leaves were one heart.

[0049] Genomic DNA was extracted for each group of biological material samples, and the specific operations were as follows:

[0050] Add 2 steel balls into a 2ml centrifuge tube, tear off about 1.5cm le...

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Abstract

The invention belongs to the technical field of melon cultivation, and specifically relates to a patent application for a triple PCR identification method for identifying the purity of melon seeds. The method is used for the identification of the purity of the hybrid species, and specifically includes the steps of extracting the DNA of the material to be tested, performing triple PCR amplification, electrophoresis detection, analysis and evaluation, and the like. The triple PCR method for quickly identifying the purity of melon seeds provided by the present application can not only identify multiple sites in the same reaction at the same time, thereby shortening the working time, but also reducing the consumption of reagent consumables by multiples. When the method of the present invention is used to identify the purity of melon seeds, it can effectively overcome the slow defect of traditional single PCR in the identification of seed purity, and has the characteristics of short time, good specificity, high accuracy, low cost and wide applicability. The purity of the melon seed material can be known simply and quickly, so that the follow-up germplasm resource analysis and breeding work can be carried out more targetedly.

Description

technical field [0001] The invention belongs to the technical field of melon cultivation, and specifically relates to a patent application for a triple PCR identification method for identifying the purity of melon seeds. Background technique [0002] melon( Cucumis melo L) for Cucurbitaceae ( Cucurbitaceae ) Melon ( Cucumis ) annual herbaceous vine originating from India and its adjoining areas. my country is a major producer of melons. In 2015, the cultivated area of ​​melons in my country reached 460,900 hectares, with an output of 15.271 million tons. [0003] During the cultivation of muskmelons, the purity of varieties is an important prerequisite to ensure the consistency of muskmelon yield and management measures. Therefore, testing the purity of varieties is an important prerequisite for related cultivation, breeding and other work. In the prior art, the identification of seed purity is a relatively heavy task, especially the rich variation phenotypes of melon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858C12Q1/6895
CPCC12Q1/6858C12Q1/6895C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2565/125
Inventor 杨路明马长生刘东明胡倩梅朱华玉胡建斌
Owner HENAN AGRICULTURAL UNIVERSITY
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