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Use of microrna precursors as drugs for inducing CD34-positive adult stem cell expansion

A technology of adult stem cells and positive cells, which is applied in the field of using microRNA precursors as drugs to induce the proliferation of CD34-positive adult stem cells, which can solve problems such as too high cost and high inefficiency

Inactive Publication Date: 2018-08-21
林希龙 +2
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Furthermore, pre-miRNA / shRNA sizes are approximately 70-85 nucleotides long, which are too large and costly to be produced by the RNA synthesis machinery

Method used

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  • Use of microrna precursors as drugs for inducing CD34-positive adult stem cell expansion
  • Use of microrna precursors as drugs for inducing CD34-positive adult stem cell expansion
  • Use of microrna precursors as drugs for inducing CD34-positive adult stem cell expansion

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[0128] In the following experimental disclosures, the following abbreviations apply: M (molar concentration); mM (millimolar concentration); μm (micromolar); mol (mole); pmol (picomolar); ; μg (microgram); ng (nanogram); L (liter); ml (milliliter); μl (microliter); ℃ (degrees Celsius); RNA (ribonucleic acid); DNA (deoxyribose nucleic acid); dNTP (three Deoxyribonucleotide phosphate); PBS (phosphate-buffered saline); NaCl (sodium chloride); HEPES (N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid); HBS ( HEPES buffered saline); SDS (sodium dodecyl sulfate); Tris-HCl (tris-hydroxymethylaminomethane-hydrochloride); ATCC (American Type Culture Collection, Rockville, MD); hESC (human embryonic stem cells); and iPSCs (induced pluripotent stem cells).

[0129] 1. Bacterial cell culture and chemical treatment

[0130] E. coli DH5α-competent cells were obtained as part of a z-competent E. coli transformation kit (Zymo Research, Irvine, CA), and subsequently cultured with 5 μg of a p...

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Abstract

This invention generally relates to a composition and method for inducing the expansion and / or regeneration of CD34-positive adult stem cell population, which is useful for developing drugs / vaccines and / or therapies against a variety of ageing-related degenerative diseases in humans. Also, the present invention teaches the production and purification methods required for making high quality and high quantity of small hairpin-like RNA (shRNA) compositions, such as microRNA precursors (pri-miRNA and pre-miRNA) and short interfering RNAs (siRNA), which are useful for treating human ageing-relateddiseases, such as, but not limited, Alzheimer's diseases, Parkinson's diseases, osteoporosis, diabetes, and cancers. The resulting shRNAs, preferably pre-miRNAs, are useful for developing therapeuticdrugs against human degenerative diseases, particularly through a mechanism to induce CD34-positive stem cell expansion and / or regeneration.

Description

[0001] Inventors: Lin Xilong (Arcadia, California), Zhang Qinci (Cerritos, California), Wu Tangxi (Arcadia, California) [0002] priority [0003] The present application claims priority to US Provisional Application No. 62 / 262,280, filed December 2, 2015, entitled "miR-302 Attenuates Aβ-Induced Neurotoxicity by Activating Akt Signaling." In addition, this invention claims priority to U.S. Patent Application No. 15 / 048,964, filed February 19, 2016, entitled "Use of miR-302 Precursors as Compositions and Methods for Treating Alzheimer's Disease" . In addition, the present invention claims priority to US Patent Application No. 15 / 167,226, filed May 27, 2016, entitled "Use of MicroRNA Precursors for Inducing CD34-Positive Adult Stem Cell Expansion." technical field [0004] The present invention generally relates to a composition and method for inducing proliferation and / or regeneration of CD34 cells using small hairpin RNA (shRNA) molecules, preferably microRNA precursors (pre...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K31/713C12N5/10C12N5/074A61P25/28A61P39/00
CPCA61P17/02A61P25/28A61P35/00A61P39/00A61P43/00C12N5/0647C12N15/113C12N2310/141C12N2310/51C12N2310/531C12N2320/32C12N2330/51C12N2501/65
Inventor 林希龙张钦次吴堂熙
Owner 林希龙
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