Microcarrier biological reaction tank and method for culturing porcine circovirus type II by microcarrier biological reaction tank

Abstract

The invention discloses a microcarrier biological reaction tank and a method for culturing porcine circovirus type II by the microcarrier biological reaction tank. An interlayer is arranged on the side wall of a tank body; heating tubes are arranged in the interlayer for heating jacket water in the interlayer so as to heat liquid in the tank body; a telescopic pipe in the tank body is used for sampling; the sampling position can be adjusted according to the amount of culture carriers, and the convenience in operation is realized; an observation pipe equipped with a transparent observation areais connected with the inner part of the tank body; a filtering gas head and a switch valve body which correspond to each other are matched for enabling carrier cells to flow into a transparent tube;cell states are observed at any time under a microscope; in addition, residual carriers are returned into the tank after observation so as to avoid waste; a carrier filter screen is arranged on the bottom side face of the reaction tank and is matched with a ventilation pipe, so that the blockage of the filter screen by the carriers when a final antigen removes the carriers during harvesting is avoided. The microcarrier biological reaction tank disclosed by the invention has the advantages that a culture device of the microcarrier biological reaction tank is improved; real-time observation of cells in the tank is facilitated; a waste solution is conveniently discharged; the carriers are favorably filtered; the efficiency is improved and 24-hour cell density is improved; cells with better states are cultured.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microcarrier bioreactor and a method for cultivating porcine circovirus type 2. Background technique [0002] The heating method of the traditional microcarrier bioreactor tank for the liquid in the tank is electric blanket heating. By heating the electric blanket, the electric blanket is attached to the outside of the reaction tank wall, and the liquid in the tank is heated by heat conduction. When the heating is just started, the electric blanket The temperature is very high, directly contacting the surface of the tank body, causing the temperature on the wall in the tank to be too high, which will affect the cell attachment and growth on the carrier in the tank. [0003] The length of the sampling tube in the tank is fixed. When the cells in the tank are cultured for 24 hours, the liquid needs to be changed, the nutrient solution in the tank is drained, and then new nutrient sol...

AI Technical Summary

Problems solved by technology

[0002] The heating method of the traditional microcarrier bioreactor tank for the liquid in the tank is electric blanket heating. By heating the electric blanket, the electric blanket is attached to the outside of the reaction tank wall, and the liquid in the tank is heated by heat conduction. When the heating is just started, the electric blanket The temperature is very high, directly contacting the surface of the tank, causing the temperature on the wall of the tank to be too high, which will affect the cell attachment and growth on the carrier in the tank

[0003] The length of the sampling tube in the tank is fixed. When the cells in the tank are cultured for 24 hours, the liquid needs to be changed, the nutrient solution in the tank is drained, and then new nutrient solution is added. When the nutrient solution in the tank is drained, it needs to be assembled and sterilized in the reaction tank Before adjusting the length of the sampling tube, the adjusted length can only be estimated according to the amount of carrier we use, and the adjustment in advance is not accurate, the liquid cannot be completely drained or the carrier is discharged, and part of the carrier is lost

[0004] When observing the cells on the carrier in the tank, it is necessary to take the carrier cells out of the tank for observation. The operation is cumbersome and affects the actual state of the observed cells. When harvesting the final antigen, all the culture medium including the carrier needs to be discharged out of the tank, and then passed through It is time-consuming and labor-intensive to filter the mixture of the harvested antigen and the carrier with a filter. The carrier cells are taken out of the tank for observation. On the one hand, the carrier cells are wasted. On the other hand, some changes will occur after the cells are taken out, and the observed cell state is biased.

Images