Composition having efficacy of delaying muscle attenuation and enhancing immunity
A technology to enhance immunity and muscle attenuation, applied in food science, function of food ingredients, food ingredients containing vitamins, etc., to promote cholesterol metabolism, improve the number of intestinal probiotics, good specific and non-specific immune regulation Effect
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[0036] 1.1.1 Preparation of mouse splenocyte suspension
[0037] Mice (Balb / c) were killed by neck pulling, soaked in 75% ethanol for 5 minutes for disinfection, and the intact spleen was aseptically isolated, and the floating blood was washed away with PBS (phosphate buffer saline), and the connective tissue and fat components were stripped off. Draw about 5mL of PBS (phosphate buffered saline) into the syringe, gently insert it into the spleen and blow out the cells, repeat the operation several times until the splenic adventitia is transparent, cut the remaining spleen tissue into small pieces of 1 mm in size, cut them into small pieces, and place them in 200 liters of water on a small beaker. Mesh stainless steel filter, grind with a syringe needle core, wash with PBS (phosphate buffer saline), collect the washing liquid into a centrifuge tube, centrifuge at 1200rpm for 5min, discard the supernatant, add 3mL red blood cell lysate, let stand for 2min, add 10mL PBS (Phosphat...
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