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A cationic liposome modified with hyaluronic acid, preparation method and application thereof

A technology of hyaluronic acid modification and hyaluronic acid, which is applied in the directions of liposome delivery, medical preparations of inactive ingredients, pharmaceutical formulations, etc. Low toxicity, good stability, not easy to oxidize

Active Publication Date: 2021-08-31
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, surgical treatment has certain risks and will cause certain irreversible damage to the surrounding tissues of the lesion.
However, traditional drug therapy has the defects of treating the symptoms but not the root cause and many side effects, and because it cannot fundamentally solve the disease, it can only be controlled by suppression, which leads to frequent and long-term medication for patients, which increases the economic burden

Method used

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  • A cationic liposome modified with hyaluronic acid, preparation method and application thereof
  • A cationic liposome modified with hyaluronic acid, preparation method and application thereof
  • A cationic liposome modified with hyaluronic acid, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0029] Prescription composition: see Table 1

[0030] Preparation method: (1) mix Tween 80 and squalene in different prescription amounts, and completely dissolve them in absolute ethanol; (2) inject the ethanol solution into a buffered saline solution at a temperature of 40-80°C at a uniform speed, and Stir for 30-50nin, and then ultrafilter 1-3 times under the ultrafiltration membrane to obtain the liposome. The particle size, PDI and potential of liposomes with different molar ratios were measured with a Malvern particle size analyzer. See Table 1.

[0031] Table 1

[0032]

[0033] Compared with Example 1 and Example 2, Example 3 reduces the amount of auxiliary phospholipids used in the composition of the prescription. In comparison, the samples added with auxiliary phospholipids in Examples 1 and 2 had a cloudy appearance, while Example 3 appeared clearer with obvious blue opalescence. Compared with Example 3 and Example 4, Example 3 increases the amount of auxilia...

Embodiment 6

[0035]

[0036]

[0037] Preparation method: (1) mix the prescribed amount of Tween 80 and cholesterol, and completely dissolve it in absolute ethanol; (2) inject the ethanol solution into the buffered saline solution at a temperature of 40-80°C at a uniform speed, and stir for 30 -50nin, and then ultrafiltration under the ultrafiltration membrane for 1-3 times to obtain liposomes. The particle size, PDI and potential of the liposomes were measured with a Malvern particle size analyzer, and the results are shown in Table 2.

[0038] Table 2

[0039]

Embodiment 7

[0041]

[0042] Preparation method: (1) mix the prescribed amount of Span 80 and squalene, and completely dissolve it in absolute ethanol; (2) inject the ethanol solution into the buffered saline solution at a temperature of 40-80°C at a uniform speed, and Stir for 30-50nin, and then perform ultrafiltration under the ultrafiltration membrane for 1-3 times to obtain liposomes. The particle size, PDI and potential of the liposomes were measured with a Malvern particle size analyzer, and the results are shown in Table 3.

[0043] table 3

[0044]

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PUM

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Abstract

The invention discloses a cationic liposome modified by hyaluronic acid, a preparation method and an application thereof. The present invention uses non-ionic surfactant, auxiliary phospholipid and cationic phospholipid as the main film-forming materials, and the outer layer wraps and modifies hyaluronic acid; wherein, the non-ionic surfactant is Tween 80 or Span 80, and the auxiliary phospholipid is squalene or cholesterol. The beneficial effect of the present invention is that: the hyaluronic acid-modified cationic liposome gene transfection efficiency of the present invention is high, and it can be specifically targeted to the retinal layer of the eye as an eye gene delivery carrier.

Description

technical field [0001] The invention relates to the technical field of liposome and gene delivery, in particular to a hyaluronic acid-modified cationic liposome, a preparation method and application thereof. Background technique [0002] Among eye diseases, fundus disease is a common and difficult-to-treat type of eye disease in clinical practice. Fundus-related diseases, such as diabetic retinopathy and non-glaucoma optic nerve damage, have a greater relationship with mortality. At present, fundus diseases seriously affect people's quality of life. At present, the treatment of fundus diseases mainly includes surgical treatment and drug treatment. Generally speaking, surgical treatment has certain risks and will cause certain irreversible damage to the surrounding tissues of the lesion. However, traditional drug therapy has the disadvantages of treating the symptoms but not the root cause, and having many side effects, and because it cannot fundamentally solve the disease...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K48/00A61K47/36A61K47/06A61P27/02
CPCA61K9/0048A61K9/1271A61K47/06A61K47/36A61K48/0008A61P27/02
Inventor 甘莉秦艳梅张华田永丰刘阳
Owner SHANGHAI INST OF TECH
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