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Intestinal probiotic agent for feeding and production method thereof

A technology of intestinal probiotics and production methods, applied in application, animal feed, animal feed, etc., can solve the problems of limiting the application of liquid bacterial agents, uneven dispersion of bacteria, high temperature mortality, etc., to improve the survival rate of bacteria , good encapsulation performance and high survival rate

Inactive Publication Date: 2018-09-04
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Probiotics are generally in two forms: liquid bacterial agents and solid powders: liquid bacterial agents have high microbial activity and rich metabolites, but have the disadvantages of being intolerant to storage and inconvenient transportation, which limit the application of liquid bacterial agents; and solid powders are usually There are problems of high production cost, uneven dispersion of bacteria and high mortality due to high temperature during the preparation process

Method used

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  • Intestinal probiotic agent for feeding and production method thereof

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Effect test

Embodiment 1

[0032] A production method for feeding intestinal probiotics, comprising the following steps:

[0033] (1) Prepare a high-density probiotic bacterium fermentation broth, the probiotics can be Lactobacillus, Streptococcus faecalis, Bacillus, Bifidobacterium, etc.;

[0034] (2) Stirring and mixing the high-density probiotic cell fermentation liquid and the microporous cornstarch to obtain the first mixed liquid;

[0035] (3) Add the thalline coating and the thermal protection agent to the mixed solution of step (2), stir to obtain the second mixed solution, the thalline coating and the thermal protection agent are lactose, trehalose, sucrose, gum arabic, yellow Any four or more of raw gum, guar gum, skimmed milk powder, β-cyclodextrin, monosodium glutamate, glycerin, and Tween 80;

[0036] (4) Drying the second mixed liquid by spray drying to obtain the intestinal probiotics for feeding.

[0037] specifically:

[0038] The probiotic of this example is Lactobacillus, and the s...

Embodiment 2

[0046] The Bacillus subtilis (Bacillus subtilis) ACCC 04396 selected in this example was purchased from the China Agricultural Microorganism Culture Collection and Management Center.

[0047] The production method of the intestinal probiotics for feeding of the present embodiment comprises the following steps:

[0048] (a) Preparation of fermented bacteria liquid, using beef extract peptone liquid medium and digitally controlled fermentation tank to produce high-density Bacillus subtilis fermentation liquid (number of Bacillus subtilis spores > 1 × 10 10 cfu / ml).

[0049] (b) Stir and mix the Bacillus subtilis fermentation broth with the microporous cornstarch for 20 minutes, and use 60 kg of microporous cornstarch in every 1 ton of fermentation broth.

[0050] (c) Use thalline coating and thermal protection agent in every 1 ton of Bacillus subtilis fermented liquid and consist of: maltodextrin 25kg, trehalose 10kg, gum arabic 1.5kg, skimmed milk powder 5kg, β-cyclodextrin 3k...

Embodiment 3

[0056] This embodiment is basically the same as Example 1, the difference is that in the step (b) of the present embodiment, the Enterococcus faecalis fermentation broth and the microporous cornstarch were stirred and mixed for 30min, and the microporous cornstarch was used in every 1 ton of fermentation broth 100kg.

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Abstract

The invention relates to an intestinal probiotic agent for feeding and a production method thereof. The method comprises the following steps: (1) preparing a high-density probiotic thallus fermentation liquid; (2) mixing the high-density probiotic thallus fermentation liquid with microporous corn starch with stirring so as to obtain a first mixed solution; (3) adding a thallus coating and a thermal protection agent into the mixed solution of the step (2), and conducting stirring to obtain a second mixed solution; and (4) carrying out drying treatment on the second mixed solution by using spraydrying to obtain the intestinal probiotic agent for feeding. Compared with the prior art, the intestinal probiotic solid powder agent produced by the method is uniform in coating particle size, goodin sacculation performance, high in survival rate, good in stability, low in production cost, convenient for storage and transportation and long in shelf life, and can be used for the production of aplurality of common probiotic agents. The coating can effectively reduce the activity loss of probiotics in the oral cavity, esophagus and stomach of animals.

Description

technical field [0001] The invention relates to the field of production of probiotics for feeding, in particular to a probiotics for feeding intestinal tract and a production method thereof. Background technique [0002] There is a huge microbial flora in the intestinal tract of animals. Various flora are interdependent and mutually restrictive, and are in a relatively balanced state. They form the largest micro-ecological environment in the body and become a natural line of defense for maintaining animal health. The microecology in the intestinal tract of healthy animals is in a balanced state, and the beneficial microorganisms occupy the absolute majority and control the entire ecosystem. Once the beneficial microbial population is destroyed and loses its dominant position, it will lead to an imbalance in the intestinal microecology and cause animal disease. [0003] In order to cope with the imbalance of intestinal microecology, many farmers use antibiotics to prevent and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/163
CPCA23K10/18A23K20/163
Inventor 李浩覃智斌赖水明柒启恩李炯明肖俊峰陈志敏傅心锋吴有林
Owner FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
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