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Integrated bone-cartilage repair scaffold and preparation method thereof

A cartilage repair and repair layer technology, used in prosthesis, tissue regeneration, medical science and other directions, can solve the problems of difficult to ensure long-term integration, loss, and peeling of cartilage and bone layer, to prevent vascular invasion and cell migration, The effect of promoting repair and good cell function

Active Publication Date: 2020-10-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, CelesteScotti et al. proposed that it is difficult to ensure the long-term integration between cartilage and bone layer only through chemical or physical combination, especially when there is no new extracellular matrix formed on the interface to fill the loss of material degradation, after a long time, Delamination of the two layers of cartilage and subchondral bone may occur, and the function of the interface layer to prevent blood vessel invasion may also be lost

Method used

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  • Integrated bone-cartilage repair scaffold and preparation method thereof
  • Integrated bone-cartilage repair scaffold and preparation method thereof
  • Integrated bone-cartilage repair scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] In this embodiment, mercapto-hyaluronic acid (HA-SH) is prepared, and the steps are as follows:

[0035] (1) Dissolve sodium hyaluronate with a molecular weight of 0.3MDa in deionized water, add N-hydroxysuccinimide (NHS), fully dissolve, and then add 1-ethyl-(3-dimethylaminopropyl ) carbodiimide hydrochloride (EDC HCl), fully dissolved, adjust the pH value of the mixture to 4.75 with 1mol / L NaOH solution and 1mol / L HCl solution, react at room temperature for 2h, and then add cysteine Ammonia hydrochloride (CSH·HCl) solution was reacted at room temperature for 24 h, and then the pH value of the reaction solution was adjusted to 8.5 with 1 mol / L NaOH solution, and dithiothreitol (DTT) solution was added to react at room temperature for 12 h.

[0036] In this step, sodium hyaluronate, N-hydroxysuccinimide (NHS), 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), semi The molar ratio of cystine hydrochloride (CSH·HCl) to dithiothreitol (DTT) is 1:2:3:2:...

Embodiment 2

[0040] In this example, a blank integrated bone-cartilage repair scaffold not containing chondrocytes and bone marrow mesenchymal stem cells was prepared, and the steps were as follows:

[0041] (1) Place the formed porous biphasic calcium phosphate ceramics (porous BCP ceramics) in a cylindrical mold with a diameter of 4 mm and a height of 5 mm. The porous BCP ceramics are composed of hydroxyapatite (HA) and β-triphosphate Composed of calcium (β-TCP), the mass ratio of HA to β-TCP is 2:8, and the porosity is about 70%. The formed porous BCP ceramic is a cylindrical block with a diameter of 4mm and a height of 2mm. The formed porous BCP ceramic It is placed on the closed end of the cylindrical mould, coaxially with the mould.

[0042] (2) Dissolve the HA-SH prepared in Example 1 with α-MEM medium, adjust the pH value to 7.2~7.4 with 1mol / L NaOH solution under ice bath, obtain 3.0wt% HA-SH solution, and immediately The HA-SH solution is injected above the shaped porous BCP cer...

Embodiment 3

[0045] In this example, to prepare an integrated bone-cartilage repair scaffold, the steps are as follows:

[0046] (1) Place the formed porous BCP ceramics in a cylindrical mold with a diameter of 4 mm and a height of 5 mm. The porous BCP ceramics are composed of HA and β-TCP, and the mass ratio of HA to β-TCP is 2:8. The porosity is about 70%. The shaped porous BCP ceramics is a cylindrical block with a diameter of 4 mm and a height of 2 mm. The shaped porous BCP ceramics are placed on the closed end of the cylindrical mold coaxially with the mold.

[0047] (2) Dissolve the HA-SH prepared in Example 1 with α-MEM medium, adjust the pH value to 7.2-7.4 with 1mol / L NaOH solution under ice bath, add Nile red dye and mix well to obtain HA-SH Concentration of 3.0wt% HA-SH-dye mixed solution, the HA-SH-dye mixed solution was injected immediately above the molded porous BCP ceramics in the mold, after the HA-SH-dye mixed solution was injected into the mold, part of the HA- The SH-d...

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Abstract

The invention provides an integrated bone-cartilage repair scaffold. The integrated bone-cartilage repair scaffold is of an integrated structure composed of a subchondral bone repair layer, a middlelayer and a cartilage repair layer, wherein the subchondral bone repair layer is fashioned porous calcium phosphate biological ceramic, the middle layer is sulfydryl-hyaluronic acid hydrogel, the cartilage repair layer is composed of I-type collagen hydrogel and chondrocytes or mesenchymal stem cells, and the middle layer is located between the subchondral bone repair layer and the cartilage repair layer and in a porous structure of the fashioned porous calcium phosphate biological ceramic to separate the subchondral bone repair layer from the cartilage repair layer. The invention further provides a preparation method of the integrated bone-cartilage repair scaffold. The repair scaffold can prevent blood vessels from invading a cartilage layer, so that the tightness and the stability of connection between a bone layer and the cartilage layer are improved, biological bonding of the bone layer and the cartilage layer is achieved, and thus a good repair effect is achieved.

Description

technical field [0001] The invention belongs to the field of osteochondral repair brackets, and relates to a bracket with an integrated repair articular cartilage, subchondral bone and interface layer at the joint between them and a preparation method thereof. Background technique [0002] Bone / cartilage damage can be caused by sports injuries, accidents or disease. Osteochondral defects contain a variety of structurally and functionally distinct tissues, of which the three main types are: articular cartilage responsible for lubrication and friction reduction, subchondral bone for load bearing, and two Different environment and able to prevent blood vessels from invading the calcified layer of the cartilage layer. Because cartilage does not contain blood vessels or nerves, whereas subchondral bone contains unmyelinated nerve endings, when joint disease produces pain, the subchondral bone may have been exposed or damaged. A survey found that more than 60% of arthritic patie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/56A61L27/52
CPCA61L27/12A61L27/20A61L27/24A61L27/3817A61L27/3834A61L27/52A61L27/56A61L2430/02A61L2430/06C08L5/08
Inventor 孙勇樊渝江蔡涵旭张兴栋
Owner SICHUAN UNIV
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