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Tissue culture propagation method of alum root kimono

A technology of kimono and alum root, which is applied in the field of tissue culture and propagation of the important ornamental plant alum root "kimono", which can solve the problems of limited and large difference in variety characteristics, and achieve the effect of improving the reproduction coefficient

Inactive Publication Date: 2018-09-07
贵州思源农旅综合开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production, the types of alum root that can be propagated by sowing are relatively limited. CN201710118920.7 A culture medium and culture method for tissue culture and rapid propagation of alum root leaves proposes a method of using leaves as explants for tissue culture propagation The method, because there are many varieties in the alum root, and the characteristics of each variety are quite different, so it cannot be fully applied to the rapid propagation of all alum roots, and there is no report on the method of tissue culture propagation using leaves for this new "kimono" variety

Method used

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  • Tissue culture propagation method of alum root kimono
  • Tissue culture propagation method of alum root kimono
  • Tissue culture propagation method of alum root kimono

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Experimental program
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Embodiment 1

[0024] The tissue culture of alum root "kimono" is carried out according to the following steps:

[0025] 1. Preparation of culture medium: configure induction medium MS medium MS+1mg / L KT+0.2mg / L TDZ+1mg / LIBA, sucrose 28g / L, agar 7g / L, pH6.0; configure differentiation culture: MS +1mg / L6-BA+0.5mg / LKT+0.1mg / L IBA, sucrose 28g / L, agar 7g / L, pH6.0; prepare strong seedling and rooting medium: MS+0.3mg / L IBA+0.5mg / LNAA, sucrose 30g / L, agar 7g / L, adjust pH 6.0;

[0026] 2. Specific operation steps:

[0027] (1) Collection of explants: alum root "kimono" is one of the more popular alum root varieties on the market, and the alum root "kimono" used in the present invention is purchased from the Yunnan Provincial Academy of Agricultural Sciences. The disease-free leaves were picked from the mother plant of Kimono;

[0028] (2) Callus induction: the leaves obtained after the sterilization process were blotted dry on the surface with sterile filter paper on the ultra-clean table, cut...

Embodiment 2

[0032] Put the alum root seedlings that need to be cultured in the greenhouse for 1 month, water them with water every day, take the disease-free leaves of the alum root "kimono", wash them under running water for 12 hours, and then set aside;

[0033] 1) Sterilization of explants: take the prepared leaves on a sterile ultra-clean table, disinfect with 75% alcohol for 20 seconds, rinse with sterile water for 3-5 times, disinfect with 0.1% mercuric chloride for 9 minutes, use Rinse with sterile water 3-5 times for later use;

[0034] 2) Callus induction: the induction medium is MS+1mg / L KT+0.2mg / L TDZ+1mg / L IBA, sucrose 28g / L, agar 7g / L, pH6.0, dark culture for 5 days and light culture 30-40 days, the light and dark time is 12h / 12h. The terminal buds obtained after the sterilization process were blotted dry on the surface with sterile filter paper on the ultra-clean table, cut into small pieces of 1cm*1cm with sterile curved scissors, inoculated into the induction medium, and ...

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Abstract

The invention discloses a tissue culture propagation method of alum root kimono. The leaves of alum roots are used as explants, and the method comprises a series of steps of carrying out disinfectionon the explants, inducing clustered shoots, subculturing and strengthening seedlings, and rooting outside a bottle; the problem of low germination rate of the new variety of alum root kimono seeds during seeding is effectively solved; the excellent leaf color and traits of the female parent are maintained, and the purpose of rapid production and propagation is achieved. The tissue culture rapid propagation method provided by the invention is suitable for tissue culture rapid propagation of the saxifragaceae alum root kimono, and belongs to the technical field of a plant biotechnology. Within one month, the propagation coefficient is 8-10, the rooting rate is 95%, and the transplanting survival rate is 95-98%; the method greatly improves the propagation coefficient of the alum root kimono;an effective propagation method is provided for rapidly expanding the market share by the variety.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a plant tissue culture propagation method, in particular to a tissue culture propagation method of an important ornamental plant alum root "kimono". Background technique [0002] Alumroot "kimono" is a new variety obtained by interspecific hybridization of the genus Alumroot in the family Saxifragaceae. It is a perennial cold-resistant herbaceous flower with shallow roots and low temperature resistance of -34°C. It has high ornamental value. In gardens, it is mostly used for understory flower borders, ground covers, garden greening, etc. There are many varieties of alum roots, some varieties have strong tillering ability, while others have weak tillering ability. In actual production, the types of alum root that can be propagated by sowing are relatively limited. CN201710118920.7 A culture medium and culture method for tissue culture and rapid propagation of alum root le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 田晓玲路登宇何娇朱强马永鹏刘鸿超
Owner 贵州思源农旅综合开发有限公司