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Method for rapidly propagating morchella cultispecies by using liquid strains

A morel culture, liquid strain technology, applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problem that the liquid strain is not suitable for sowing, and achieve the effect of improving the preparation efficiency

Inactive Publication Date: 2018-09-14
BEIJING AGRI TECH PROMOTION STATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For morels, liquid strains are not suitable for direct seeding due to the need to sow the strains in the soil

Method used

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Examples

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Embodiment Construction

[0021] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0022] A kind of method that utilizes liquid bacterial classification to multiply hickory chick cultivar fast comprises the following steps of the present invention:

[0023] 1), prepare the medium of hickory chick liquid original seed, the substratum of hickory chick liquid original seed is composed as follows: potato 200g, glucose 20g, dipotassium hydrogen phosphate 2g, magnesium sulfate 1g, yeast powder 1g, peptone 2g, vitamin B 1 0.5g;

[0024] 2), dig out the bud eyes of the potatoes, peel them, cut them into small pieces of 1cm square, cook on low heat for 20 minutes, then use 8 layers of gauze to filter, and take the filtrate;

[0025] 3), weigh glucose, dipotassium hydrogen phosphate, magnesium s...

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Abstract

The invention relates to the technical field of morchella strain preparation, in particular to a method for rapidly propagating morchella cultispecies by using liquid strains. The method comprises thefollowing steps that 1) a culture medium of morchella liquid protospecies is prepared, and the culture medium of the morchella liquid protospecies is prepared from 200 grams of potato, 20 grams of glucose, 2 grams of dipotassium phosphate, 1 gram of magnesium sulfate, 1 gram of yeast powder, 2 grams of peptone and 10.5 grams of vitamin B; 2) bud eyes of the potato is dug out, the potato is peeledand cut into 1centimerter square of small blocks, cooking on low heat is carried out for 20 minutes, and then eight layers of gauzes are used to perform filtering to extract filtrate; 3) the glucose,the dipotassium phosphate, the magnesium sulfate, the yeast powder, the peptone and the vitamin are weighed in proportion and added into the filtrate, constant volume is carried out to 1000 milliliters with distilled water, and a pH value is adjusted to 7.0; 4) 100 milliliters of the prepared liquid culture medium in step 3) is put into a 250 milliliters of shake flask, sealing is carried out with sealing film, and sterilization is carried out at 121 DEG C for 20 minutes; and 5) the sterilized culture medium is cooled to the indoor temperature.

Description

technical field [0001] The invention relates to the technical field of hickory chick strain preparation, in particular to a method for quickly multiplying hickory chick cultivars by using liquid strains. Background technique [0002] As we all know, with the advancement of science and technology, some species of Morchella have been artificially cultivated and become an emerging mushroom species with great potential. In recent years, with the gradual deepening of the research on the artificial cultivation technology of morels, the planting area of ​​morels in the country continued to increase from 2012 to 2016. According to statistics, the cultivated area reached 23,400 mu in 2016, and the large-scale planting area gradually expanded from the main production area of ​​Sichuan Promoted to Hubei, Henan, Hebei and other provinces, it has become the most popular cultivar in the edible fungus industry. [0003] Morels are planted on a large scale, the sowing period is relatively ...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 贺国强魏金康邓德江赵海康吴尚军胡晓艳
Owner BEIJING AGRI TECH PROMOTION STATION