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Prefabricated test powder for rapidly preparing nucleic acid electrophoretic buffer solution based on boric acid and use method thereof

An electrophoresis buffer, boric acid technology, applied in the field of biological analysis, can solve the problem of wasting manpower and time, and achieve the effect of simple and fast use process, reducing preparation time, and improving the efficiency of electrophoresis experiments

Inactive Publication Date: 2018-09-18
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with TAE buffer, TBE buffer has better nucleic acid stability and longer electrophoretic life, but whether it is TAE or TBE buffer, it needs to be prepared by the experimenter, including weighing a number of drugs and adjusting the pH value, etc. steps, wasting a lot of manpower and time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Prepare prefabricated test powder based on the preparation of 1L 1× concentration of nucleic acid electrophoresis buffer, each test powder contains 10.8g of Tris base, 5.5g of boric acid, 0.744g of Na 2 EDTA·2H 2 O, 3.53 g of KH 2 PO 4 . Take a portion of the test powder and mix it fully with 1L of deionized water to obtain a 1× concentration electrophoresis buffer (Tris base 90mmol / L, boric acid 90mmol / L, Na 2 EDTA 2mmol / L, KH 2 PO 4 26mmol / L, pH value 8.02), after 5 hours of electrophoresis, the pH value of the positive electrode in the electrophoresis tank is 8.0, the pH value of the negative electrode is 8.4, the nucleic acid sample is stable, and the ultraviolet imaging is good.

Embodiment 2

[0019] Slightly adjust the test powder formula in Example 1, based on the preparation of 1L 1× concentration of electrophoresis buffer, each prefabricated test powder contains 10.8g of Tris base, 5.5g of boric acid, 0.372g of Na 2 EDTA·2H 2 O, 3.4 g of KH 2 PO 4 . Take a portion of the test powder and mix it fully with 1L of deionized water to obtain a 1× concentration electrophoresis buffer (Tris base 90mmol / L, boric acid 90mmol / L, Na 2 EDTA 1mmol / L, KH 2 PO 4 25mmol / L, pH value 8.03), after 5 hours of electrophoresis, the pH value of the positive electrode in the electrophoresis tank is 8.1, the pH value of the negative electrode is 8.3, the nucleic acid sample is stable, and the ultraviolet imaging is good.

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PUM

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Abstract

The invention belongs to the technical field of biological analysis, and provides prefabricated test powder for rapidly preparing a nucleic acid electrophoretic buffer solution based on boric acid anda use method thereof. The prefabricated test powder comprises the following components by weight: based on preparation of 1 L of the electrophoretic buffer solution with one-time (1*) concentration,each part of the prefabricated test powder contains 9.6-12 g of Tris base, 4.89-6.11 g of boric acid, 0.186-0.744 g of Na2EDTA.2H2O and 3.40-3.67 g of KH2PO4. When in use, at room temperature, each part of the test powder is fully mixed and mutually soluble with 1 L of deionized water, and thus 1L of the electrophoretic buffer solution with 1* concentration is obtained. The steps of weighing manydrugs and adjusting the pH value of the solution in a conventional method are omitted during preparation of the electrophoretic buffer solution, so that the preparation process of the buffer solutionis completed within 5-10 minutes, and the efficiency of nucleic acid electrophoresis experiments is improved.

Description

technical field [0001] The invention belongs to the field of biological analysis, and provides a prefabricated test powder for rapid preparation of nucleic acid electrophoresis buffer in biochemical or molecular biology experimental analysis and a method for using the same. Background technique [0002] Electrophoresis detection plays an important role in the biochemical and molecular biological analysis of nucleic acids. It is one of the routine methods for nucleic acid quality and concentration detection, and an important basis for analyzing the size, mass concentration and purity of DNA and RNA fragments. Traditional nucleic acid electrophoresis mainly uses acetate buffer system TAE (Tris-Acetic acid-EDTA) or borate buffer system TBE (Tris-Boric acid-EDTA). Compared with TAE buffer, TBE buffer has better nucleic acid stability and longer electrophoretic life, but whether it is TAE or TBE buffer, it needs to be prepared by the experimenter, including weighing a number of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38G01N27/447
CPCG01N1/38G01N27/447
Inventor 徐卫平王文霞刘玉婷许建强佟慧妍穆罕默德·拉塞尔杨骁婧
Owner DALIAN UNIV OF TECH