Electrochemical test paper for rapid measurement of amylase as well as preparation and detection methods thereof

An amylase and electrochemical technology is applied in the field of electrochemical test paper for rapid measurement of amylase and its preparation and detection. Effect

Pending Publication Date: 2018-09-18
GUILIN ZHONGHUI TECH DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are many methods for the determination of serum and urine amylase activity, mainly including iodide-starch colorimetry and enzyme rate method. Fully automatic multi-parameter analyzer testing, patients need to go to a medical institution to obtain testing services, it takes a long time, and they cannot self-monitor at home

Method used

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  • Electrochemical test paper for rapid measurement of amylase as well as preparation and detection methods thereof
  • Electrochemical test paper for rapid measurement of amylase as well as preparation and detection methods thereof
  • Electrochemical test paper for rapid measurement of amylase as well as preparation and detection methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] In this example, see figure 1 , an electrochemical test paper for rapid measurement of amylase, comprising

[0057] insulating substrate 1, and

[0058] Two mutually independent detection systems arranged on the insulating substrate 1, the detection system includes a liquid guiding groove body arranged in the insulating substrate 1, and the liquid guiding groove body includes an independent first liquid guiding groove 2- 1 and the second liquid guide tank 2-2, the first liquid guide tank 2-1 is provided with the first sample inlet 3-1, and the second liquid guide tank 2-2 is provided with the second sample inlet 3-2; include

[0059] The conductive film layer is arranged on the insulating substrate 1, and the conductive film layer includes a first reference electrode 4-1, a first working electrode 4-2, and a second reference electrode 4-1 insulated from each other. 3 and the second working electrode 4-4;

[0060] Reaction film layer, the reaction film layer include...

Embodiment 2

[0093] This example illustrates the linear relationship between amylase activity and current, and the method for constructing a calibration curve using the amylase test paper prepared in Example 1.

[0094]Collect fresh venous blood, adjust hematocrit ratio to 40%, prepare amylase activity as 10 U / L, 25 U / L, 50 U / L, 100 U / L, 250 U / L, 500 U / L, 1000 U / L blood sample, the amylase test paper prepared in Example 1 is placed in a multi-parameter analyzer and a 300mV working voltage is applied at both ends of the working electrode and the reference electrode; the blood sample is added to the sampling port and automatically drawn by siphon action About 5 µL of sample enters the reaction zone 1 to react with the reagents in the first reaction membrane 5-1, and the reaction zone 2 and the second reaction membrane 5-2, and measures the current I1 of the first working electrode 4-2. The current I1 includes amylase Decompose the glucose formed by the substrate and the glucose reaction curr...

Embodiment 3

[0099] This example illustrates the use of the calibration curves constructed in Example 2 and Comparative Example 2 to measure blood samples with different amylase activities, and to illustrate the influence of different glucose concentrations on the test results.

[0100] Blood samples with different amylase activities and different glucose concentrations were collected.

[0101] Place the amylase test paper prepared in Example 1 in a matching tester and apply a working voltage of 300mV across the working electrode and the reference electrode; take a blood sample and add it to the sampling port, and automatically draw about 5 µL of the sample into the reaction area by siphon action 1 reacts with the enzyme reaction membrane 1 and the reaction zone 2 reacts with the reagent in the enzyme reaction membrane 2, and measures the current I1 of the working electrode of the first electrode system. The current I1 includes the glucose formed by amylase decomposing the substrate and the...

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Abstract

The invention discloses electrochemical test paper for rapid measurement of amylase as well as preparation and detection methods thereof. The electrochemical test paper comprises an insulating substrate, two independent detection systems arranged on the insulating substrate, a conductive film layer, a reaction film layer and a curing layer, wherein the detection system comprises a liquid guide groove body formed in the insulating substrate; the liquid guide groove body comprises a first liquid guide groove and a second liquid guide groove being independent of each other; the first liquid guidegroove is provided with a first sample inlet; the second liquid guide groove is provided with a second sample inlet; the conductive film layer is arranged on the insulating substrate; the conductivefilm layer comprises a first reference electrode, a first working electrode, a second reference electrode and a second working electrode being mutually insulated from one another; the reaction film layer comprises at least one electron mediator and at least one enzyme which reacts with a detected substance. The electrochemical test paper can be used in conjunction with a multi-parameter analyzer to rapidly and quantitatively detect amylase activity in blood or urine, and has the advantages of high sensitivity, accurate measurement results and convenience in use.

Description

technical field [0001] The invention relates to the detection field of a human body in vitro diagnostic reagent, in particular to an electrochemical test paper for rapidly measuring amylase and a preparation and detection method thereof. Background technique [0002] The full name of amylase (AMY or AMS) is 1,4-α-D-glucan hydrolase, which catalyzes the hydrolysis of starch and glycogen to produce glucose, maltose and dextrin containing α1,6-glycosidic bond branch. Amylase is mainly secreted by pancreas and salivary glands, and also contains this enzyme in lung, liver, thyroid, fat and other tissues. [0003] In patients with acute pancreatitis, pancreatic amylase overflows the pancreas, is quickly absorbed into the blood, and is excreted in the urine. Therefore, the amylase in blood and urine increases greatly. The serum amylase begins to increase 1 to 2 hours after the onset of the disease, and the specimen is most valuable at 8 to 12 hours , reached the highest peak at 24...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 梁明龙刘勤伍寿胜李中廖姗
Owner GUILIN ZHONGHUI TECH DEV
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