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Poplar somatic embryo generation and plant construction method

A technology for regenerating plants and poplars, which is applied in the field of plant tissue culture, can solve the problems of slow reproduction speed, high labor intensity, and great influence from the external environment, and achieve the effect of high repeatability and short culture period

Active Publication Date: 2018-09-28
BEIJING FORESTRY UNIVERSITY
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AI Technical Summary

Problems solved by technology

Traditional poplar seedlings are raised mainly through cuttings, grafting, root propagation and other methods, but these methods usually have disadvantages such as slow propagation speed, great influence from the external environment, low survival rate, and high labor intensity.

Method used

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  • Poplar somatic embryo generation and plant construction method
  • Poplar somatic embryo generation and plant construction method
  • Poplar somatic embryo generation and plant construction method

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Experimental program
Comparison scheme
Effect test

Embodiment 1、84

[0059] The establishment of embodiment 1,84K poplar regeneration system

[0060] figure 1 It is the process of obtaining regenerated plants with poplar leaves as explants, wherein, A is the seedling; B is the leaf; C is the induction culture; D is the callus; E is the differentiation culture; F is the rooting culture; G and H built for plants.

[0061] 1. The test material is the 84K poplar in the experimental field. When collecting the material, select the young and vigorous leaves, put them in a pre-frozen ice box, and wrap them with moist gauze to prevent water loss.

[0062] 2. The retrieved experimental materials were first washed with tap water to show the dirt, and then disinfected. Four different disinfectants were used to disinfect the materials in the whole experiment. The experimental results are shown in Table 2. The experimental results show that the disinfection effect of ethanol is better, and the residual ethanol on the material is volatile, easy to remove, a...

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Abstract

The invention discloses a poplar high efficiency somatic embryo generation and plant construction method, and more specifically provides a polar tissue culture method. The poplar somatic embryo generation and plant construction method comprises following steps: 1, an induction medium (containing 1.0 to 2.0mg / L 2,4-D and 0.1mg / L KT) is inoculated with an explants of polar for culturing so as to obtain callus; a differentiation medium is inoculated with the callus for culturing so as to obtain adventitious buds; 3, a rooting medium is inoculated with the adventitious buds for culturing so as toobtain seedlings with roots. The polar tissue culture method possesses following advantages: the culture period is short; the repeatability is high; different hormone combinations are adopted, so thatpoplar embryo generation rate is increased; and a large amount of regenerated plants can be obtained. The polar tissue culture method is capable of providing an ideal experiment system for deep research of plant cell differentiation, and an important platform for polar cell engineering, genetic engineering, and polar genetic improvement.

Description

technical field [0001] The invention belongs to the field of plant tissue culture and relates to a method for somatic embryogenesis and plant establishment of poplar. Background technique [0002] Poplar is one of the important tree species widely cultivated in the world. Because of its fast growth, high yield and strong practicability, it is well used in urban greening, timber production and ecological construction. Traditional poplar seedlings are raised mainly through cuttings, grafting, root propagation and other methods, but these methods usually have disadvantages such as slow propagation speed, great influence from the external environment, low survival rate, and high labor intensity. [0003] In recent years, with the rapid development of disciplines such as plant physiology and molecular biology, poplar tissue culture technology has become increasingly mature. As a model plant in forest tree genetics and breeding, poplar has special significance for the study of fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 林金星崔亚宁王鑫伟付春祥曹英萍吴振映
Owner BEIJING FORESTRY UNIVERSITY
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