Preparation method of sericin/agarose gel loaded with lysozyme and application thereof

Abstract

The invention belongs to the field of biological material technology, and discloses a preparation method of sericin / agarose gel loaded with lysozyme and application thereof. The method comprises the steps that silkworm cocoon is cut into pieces, high temperature and high pressure at 121 DEG C is carried out for 30 minutes, the sericin is extracted into water, then sericin solution is lyophilized to make sericin powder for preservation, and the sericin solution is obtained by dissolving the sericin powder in hot water; sericin of 2% and agarose solution of 2% (w / V) are prepared for preparationof a hydrogel, the two solutions are mixed lightly and 24 hole cell culture plate is added for gelation; and the stable hydrogel is subjected to pre-freezing for 12h at -80 DEG C, and then thestable hydrogel is subjected to freeze drying for 24h to form gel. Sericin / agarose plural gel has porosity and interconnected structure with good swelling property. Sericin / agarose / lysozyme gel is prepared bya solution soaking method. The gel has sustained lysozyme release ability, and had good antibacterial activity and excellent cytocompatibility for escherichia coli and staphylococcus aureus.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a preparation method and application of sericin / agarose gel loaded with lysozyme. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Unhealed wound skin is frequently exposed to bacteria, causing wound infection, which is biologically characterized by prolonging inflammation, interfering with re-epithelialization, impeding collagen production, and ultimately delaying wound healing. In wound care, wound dressings are an important biomedical material used to protect wounds and promote wound healing. The market for wound dressings is rapidly increasing with the increasing number of chronic diseases in the current healthcare system across the world. An ideal wound dressing should absorb wound exudate in a timely manner, allow gas exchange, and maintain the necessary moisture at the wound interfa...

AI Technical Summary

Problems solved by technology

[0004] In summary, the problems in the prior art are: Sericin is a good protein resource, but its amorphous structure makes it physically brittle and highly soluble, so it needs to be modified to develop its application potential in biomedical materials; agarose has good mechanical properties and Biocompatibility, but its cell adhesion and proliferation activity is low; hydrogel can provide a favorable growth environment for infectious bacteria, so it is impossible to use it as a wound dressing material; developed metal / metal oxide and other antibacterial Materials, which may adversely affect ecosystems during production and use

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