An imaging device and operation method for neuron activation by low-frequency pulsed ultrasound

Abstract

The invention discloses a low intensity pulsed ultrasound activated neuron imaging device and an operation method. The imaging device comprises a low intensity pulsed ultrasound exciter, an objectivetable and a microscope, wherein the low intensity pulsed ultrasound exciter is arranged above the objective table; the microscope is arranged under the objective table; the objective table is used forplacing a culture dish filled with neurons; the low intensity pulsed ultrasound exciter is used for emitting low intensity pulsed ultrasound waves into the neurons in the culture dish to perform mechanical stimulation on the neurons for generating the activation or inhibition biological effect; the microscope is used for emitting laser to the neurons in the culture dish, and the neurons in the culture dish are observed through refracted scattered light. The integrity and the survival rate of the neurons are ensured, so that the neurons can be better studied.

Description

technical field [0001] The invention relates to the technical field of medical engineering, in particular to an imaging device and an operation method for activating neurons by low-frequency pulsed ultrasound. Background technique [0002] The human visual pathway nervous system is the pathway of visual transmission. From the optic nerve, through the optic chiasm, optic tract, lateral geniculate body, and optic radiation to the cortical optic center. After the nerve cells on the retina are stimulated by light, they generate nerve impulses, which are transmitted to the visual center in the brain through the nervous system. Optic nerve damage or persistent high intraocular pressure can lead to massive death of retinal ganglion cells. At present, "optogenetics", deep brain stimulation and non-invasive "magnetogenetics" are usually used. Optogenetics and deep brain stimulation require implantation of optical fibers or metal electrodes in the brain, which will cause direct dama...

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Problems solved by technology

However, its further neurological effects have not been elucidated, mainly because researchers have not yet defined the threshold of action of low-frequency energy and time window in target tissues and target cells

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