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A method for creating extra-large panicle and wide-compatibility tetraploid rice

A tetraploid and wide-parenting technology, which is applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of direct application of autotetraploid rice, difficulty in exerting yield advantages, low fertility caused by breeding, etc. problem, to achieve the breakthrough of rice polyploid large heterosis, overcome hybrid sterility, and achieve high seed setting rate

Active Publication Date: 2021-10-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, autotetraploid rice and its interspecies hybrid F 1 Like other newly synthesized polyploids, there are many unfavorable traits. Among them, the unfavorable traits that have the greatest impact on breeding are generally low fertility and difficulty in producing yield advantages, making it difficult to directly apply autotetraploid rice in production.

Method used

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  • A method for creating extra-large panicle and wide-compatibility tetraploid rice
  • A method for creating extra-large panicle and wide-compatibility tetraploid rice
  • A method for creating extra-large panicle and wide-compatibility tetraploid rice

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 A kind of creation method of extra large panicle and wide compatibility novel tetraploid rice

[0034] A method for creating a new type of tetraploid rice with extra large panicle and broad compatibility (the process is as follows: figure 1 shown), specifically include the following steps:

[0035] 1. Screening of donor and recipient parents

[0036] Among the 4 materials including L202-4x, Bantau Nante-4x, Liaojing 944-4x and Raoping Broom-4x, one was selected to carry the embryo sac at the same time S 5 n The autotetraploid rice material with pollen fertility "neutral gene" was used as the donor male parent. Among 6 materials including Linglun-4x, Guangluai 4-4x, Yanjing 48-4x, Japonica 89-4x, Dadarbai 26-4x, Nanhai Zaoyinzhan-4x and Shennong 265, 1 wide affinity S 5 n The autotetraploid indica rice was used as the recipient parent.

[0037] through the embryo sac S 5 nIdentification of functional molecular markers and pollen fertility "neutra...

Embodiment 2

[0055] Example 2 Overcome hybrid sterility with extra-large panicle and wide-compatibility new tetraploid rice line

[0056] 1. Specific operation

[0057] The new tetraploid rice line Huaduo 8 (H7) with extra large panicle and wide compatibility constructed in Example 1 was hybridized with different types of autotetraploid rice for the first generation of hybrids (see Table 1 for hybrid combinations, identification of fertility, evaluation The effect of new materials on overcoming hybrid sterility.

[0058] 2. Results

[0059] As shown in Table 1, the seed-setting rate of the first generation of hybrid hybrids of extra-large panicle wide-compatibility new tetraploid rice and different types of autotetraploid rice can reach more than 70%, which can well overcome hybrid sterility. Such as image 3 It shows that the plant type of extra large panicle wide-compatibility new tetraploid rice and the first generation of its hybrids.

[0060] Table 1: F of a new type of tetraploid...

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Abstract

The invention discloses a method for creating a new type of tetraploid rice with extra-large panicle and wide compatibility, which comprises the following steps: using the "neutral gene" carrying pollen fertility and embryo sac wide compatibility S 5 n The autotetraploid rice is the male parent, which carries the embryo sac and is broadly compatible S 5 n F 1 Generation; F 1 F 16 For each generation, select a single plant with a main panicle length of more than 20cm and a seed setting rate of more than 50%; 17 Select the single plant with the main panicle length exceeding 30cm, the number of grains per panicle exceeding 200, and the seed setting rate exceeding 60%; continue to self-cross to F 21 In each generation, individual plants with panicle length exceeding 35cm, grain number per panicle exceeding 250, and seed setting rate exceeding 70% were selected to obtain a new type of tetraploid rice with extra large panicle and wide compatibility. The technology of the present invention can overcome the sterility of hybrids, and the seed setting rate of the first generation of hybrids in hybrid groups with different types of autotetraploid rice is normal, and it is expected to be used in a large number of autotetraploid rice groups to select strong and superior combinations and realize A breakthrough in the utilization of heterosis in polyploid rice.

Description

technical field [0001] The invention relates to the technical field of rice breeding, and more specifically, relates to a method for creating a new type of tetraploid rice with extra large panicle and broad compatibility. Background technique [0002] At present, polyploid rice is mainly autotetraploid rice, which is a new germplasm of diploid rice through artificial chromosome doubling. Polyploid rice not only provides a large amount of material basis for mutation, evolutionary adaptation and selection, but also becomes one of the important ways to promote plant evolution and breeding. Moreover, the interspecific hybrid F 1 It has strong biological advantages, contains huge potential for increasing yield, and is expected to become a new way of rice breeding in the future, so it has attracted great attention from rice researchers. [0003] However, autotetraploid rice and its interspecies hybrid F 1 Like other newly synthesized polyploids, there are many unfavorable trait...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 刘向东吴锦文俞淑红卡森陈志雄王兰
Owner SOUTH CHINA AGRI UNIV
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