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Monoecious spinach and breeding and cultivating method thereof

A technology of monoecious plants and cultivation methods, applied in the fields of monoecious spinach and its breeding and cultivation, can solve the problems of complex genetic traits of spinach, insufficient pollen quantity, high technical requirements, etc., achieve important application and scientific research value, enrich Ecotype, increasing the effect of parent material

Inactive Publication Date: 2018-10-09
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
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Problems solved by technology

[0003] Spinach is a dioecious plant. During the long-term artificial breeding process, stable female line materials have appeared. However, due to the complex genetics of spinach sex traits, it is a cross-pollinated plant, and artificial self-breeding often has a certain proportion of outcrossing. , resulting in insufficient material purity
Even if self-propagation can be carried out through female line materials, special treatment of female line materials is required during the flowering period, which requires relatively high technical requirements, and is also time-consuming, labor-intensive and labor-intensive; in addition, the amount of pollen produced by female line materials after treatment Insufficient, it is only enough for the self-breeding of the female line material, and cannot be used as the male parent for cross-pollination

Method used

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  • Monoecious spinach and breeding and cultivating method thereof

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Embodiment 1

[0026] 1. F 1 Obtaining hybrid seeds: the female line seed SP2-5-1-38 is used as the female parent and the dioecious inbred line seed SP4-10-4-29 is used as the male parent and sowed at 4°C for germination at low temperature. The male parent is sown from September to December in autumn, and the female parent is sown 15 to 20 days later than the male parent. Conventional management methods are adopted at the seedling stage. In the following year, during the flowering period of the male parent, gently shake the flowering branches to make the pollen fall on the stigma of the female parent; after flowering, pay attention to fertilizer and water management, apply more potassium and boron fertilizers, and pull out weeds in time.

[0027] 2. F 2 Seed acquisition from selfing offspring: F that will be harvested 1 Seed germination and sowing, routine management at the seedling stage, pull out the male plants at the flowering stage of the next year, give the female plants gibberellin ...

Embodiment 2

[0031] 1. F 1 Obtaining hybrid seeds: the female line seed SP2-5-1-38 is used as the female parent and the dioecious inbred line seed SP4-10-4-29 is used as the male parent and sowed at 4°C for germination at low temperature. The male parent is sown in the artificial climate room, and the female parent is sown 10 to 15 days later than the male parent. The seedling substrate is made of peat soil, perlite and vermiculite at a ratio of 3:1:1. In a nutrient bowl with a diameter of 20cm. The light intensity of the artificial climate chamber is 8000-9500Lux, the temperature is 25°C during the day, 15°C at night, the humidity is 65%, and the photoperiod is 12 / 12 hours. After 20 days of growth, adjust the photoperiod to 18 / 6 hours, and it will bloom in about 30 days. After flowering, gently shake the flower branches of the male parent plant to make the pollen fall on the stigma of the female parent; after flowering, strengthen fertilizer and water management, and apply more potassiu...

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Abstract

The invention discloses a breeding method of monoecious spinach. The breeding method of the monoecious spinach comprises the following steps: step 1, carrying out hybridization on a female seed as a female parent and a diecious seed as a male parent to obtain a F1 seed; step 2, sowing the F1 seed obtained from step 1, carrying out conventional management in a seedling stage, removing staminiferousplants in a flowering stage, treating and inducing female plants to produce pollen by using plant hormones, and carrying out selfing to obtain a F2-generation seed; and step 3, continuously inducingthe female plants to carrying out selfing to produce 5-6 generations, and screening to obtain a monoecious spinach selfing line with stable inheritance. The invention further discloses the monoeciousspinach obtained by the breeding method and a cultivating method of the monoecious spinach. By the provided breeding method of the monoecious spinach selfing line, a stable homozygous monoecious selfing line can be obtained by three years only, and the selfing line can be used for rapid selfing affinity of a spinach transgenic or mutagenic material.

Description

technical field [0001] The invention relates to the technical field of vegetable cultivation and breeding, in particular to a monoecious spinach and a breeding and cultivation method thereof. Background technique [0002] Spinach (Spinacia oleracea L.) is an annual or biennial herbaceous plant belonging to the Chenopodiaceae family of Amaranthaceae. It is one of the important green leafy vegetable economic crops. It has a short growth cycle and a high multiple cropping index. It is widely planted in the north and south of my country. my country is the largest producer and consumer of spinach in the world, with an annual output of more than 12 million tons, accounting for about 90% of the world's total output. [0003] Spinach is a dioecious plant. During the long-term artificial breeding process, stable female line materials have appeared. However, due to the complex genetics of spinach sex traits, it is a cross-pollinated plant, and artificial self-breeding often has a cert...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 蔡晓锋葛晨辉王全华徐晨曦王小丽
Owner SHANGHAI NORMAL UNIVERSITY
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