Method for producing paper mulberry tissue culture seedling

A technology for tissue culture seedlings and tree structures, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of poor quality, low survival rate, and insufficient strength of tree structure trees, and achieve the effect of improving stress resistance

Inactive Publication Date: 2018-10-12
贵州务川科华生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention intends to provide a method for producing tissue-cultured seedlings of mulberry tree, so as to s

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: a kind of method of producing mulberry tissue culture seedling, comprises the following steps:

[0024] (1) Female parent planting: plant the female parent plant in the greenhouse, strengthen fertilizer and water management, ensure the robustness of the plant, prevent and control diseases and insect pests, and reduce the microorganisms and pests attached to the plant surface;

[0025] (2) Explant selection: select plant materials such as buds, stems or leaves from healthy plants as explants;

[0026] (3) Sterilization of explants: surface sterilization of the obtained explants;

[0027] (4) Primary culture: cut the sterilized explants into 0.5cm*0.5cm*0.5cm blocks on the surface of sterile filter paper, and then insert them into the primary medium for cultivation until the explants grow out For sprouts, the primary culture medium is made by adding 2,4-dichlorophenoxyacetic acid, cytokinin, 6-benzylaminoadenine and 1-naphthaleneacetic acid to MS medium, in...

Embodiment 2

[0034] Embodiment 2: a kind of method of producing mulberry tissue culture seedling, comprises the following steps:

[0035] (1) Female parent planting: plant the female parent plant in the greenhouse, strengthen fertilizer and water management, ensure the robustness of the plant, prevent and control diseases and insect pests, and reduce the microorganisms and pests attached to the plant surface;

[0036] (2) Explant selection: select plant materials such as buds, stems or leaves from healthy plants as explants;

[0037] (3) Sterilization of explants: surface sterilization of the obtained explants;

[0038] (4) Primary culture: cut the sterilized explants into 0.5cm*0.5cm*0.5cm blocks on the surface of sterile filter paper, and then insert them into the primary medium for cultivation until the explants grow out For sprouts, the primary culture medium is made by adding 2,4-dichlorophenoxyacetic acid, cytokinin, 6-benzylaminoadenine and 1-naphthaleneacetic acid to MS medium, in...

Embodiment 3

[0045] Embodiment 3: a kind of method of producing mulberry tissue culture seedling, comprises the following steps:

[0046] (1) Female parent planting: plant the female parent plant in the greenhouse, strengthen fertilizer and water management, ensure the robustness of the plant, prevent and control diseases and insect pests, and reduce the microorganisms and pests attached to the plant surface;

[0047] (2) Explant selection: select plant materials such as buds, stems or leaves from healthy plants as explants;

[0048] (3) Sterilization of explants: surface sterilization of the obtained explants;

[0049] (4) Primary culture: cut the sterilized explants into 0.5cm*0.5cm*0.5cm blocks on the surface of sterile filter paper, and then insert them into the primary medium for cultivation until the explants grow out For sprouts, the primary culture medium is made by adding 2,4-dichlorophenoxyacetic acid, cytokinin, 6-benzylaminoadenine and 1-naphthaleneacetic acid to MS medium, in...

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Abstract

The invention discloses a method for producing a paper mulberry tissue culture seedling in the technical field of paper mulberry seedling cultivation. The method comprises (1) female parent planting,(2) explant selection, (3) explant sterilization, (4) primary culture, (5) proliferation culture, (6) seedling strengthening culture, (7) rooting culture, (8) seedling hardening and transplanting, (9)young seedling culture in an aperture disk and (10) emergence and packaging. A healthy plant is selected as an explant, which has a high-quality gene, and then is sterilized so that the resilience ofthe explants is improved. Through primary culture, value-added culture, seedling strengthening culture and rooting culture, a strong paper mulberry seedling is rapidly cultured.

Description

technical field [0001] The invention belongs to the technical field of seedling cultivation of mulberry tree, and in particular relates to a method for producing tissue cultured seedlings of mulberry tree. Background technique [0002] The mulberry tree, also known as Chu Tao, is a deciduous tree with a height of 10-20m, dark gray bark, and densely pubescent twigs. The crown is open, ovate to broadly ovate, the bark is smooth, light gray or taupe, not easy to crack, the whole plant contains latex, it is a strong positive tree species, with strong adaptability and strong stress resistance. Although the appearance of the mulberry tree is relatively rough, it has many advantages such as dense branches and leaves, resistance, fast growth, and easy reproduction. The fruit is sweet and sour and edible. It is selected as a shade tree and a shelter forest. It is a tree species with strong resistance to toxic gases and can be planted in areas with severe air pollution. [0003] The...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 冉贤
Owner 贵州务川科华生物科技有限公司
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