Preparation method and application of sulfated echinacea purpurea polysaccharide

A technology of echinacea and sulfation, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, allergic diseases, etc., can solve the lack of research on the inability to effectively regulate the immunity of animals, the ability to resist diseases, and the improvement of immune enhancement performance , Low activity of immunomodulatory drugs and other issues, to achieve good body immune regulatory function, broad application market for disease prevention and control, and significant immune regulatory effect

Active Publication Date: 2018-10-12
SOUTH CHINA AGRI UNIV
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Echinacea purpurea polysaccharides have the characteristics of general traditional Chinese medicine polysaccharides, and have been used clinically. However, the current research on echinacea purpurea polysaccharides is mostly focused on its extraction methods and additives compounded with other substances, and there is a lack of improvement in the immune enhancement performance of echinacea purpurea polysaccharides. Research shows that the obtained immunomodulatory drugs have low activity and cannot effectively regulate the immune ability of animals and the ability to resist diseases. There is a problem of lack of efficient and simple immunomodulatory drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of sulfated echinacea purpurea polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of sulfated echinacea purpurea polysaccharide, preparation steps are as follows:

[0044] S1. Preparation of chlorosulfonic acid-pyridine esterification reagent;

[0045] S2. Prepare a homogeneous solution of Echinacea purpurea polysaccharide by using purified Echinacea purpurea polysaccharide: Weigh 3 g of purified Echinacea purpurea polysaccharide and put it into an Erlenmeyer flask and add appropriate amount of N, N-dimethylformamide to prepare a homogeneous solution of Echinacea purpurea polysaccharide;

[0046] S3. Add the esterification reagent to the homogeneous solution of Echinacea purpurea polysaccharide, stir and close the reaction at 80°C for 3h, after the reaction is complete, cool to room temperature, add pre-cooled 100 mL ice water, and neutralize with 5mol / L NaOH solution to The pH value is neutral, add 3 to 4 times the volume of 80% ethanol, and let it settle for 24 hours;

[0047] S5. Take out the precipitate by suction filtration, add an appro...

Embodiment 2

[0056] The sulfated echinacea purpurea polysaccharide prepared in the above-mentioned embodiment 1 is applied to the immune regulation of chickens, and the use concentration is 2 -7 mg / mL.

Embodiment 3

[0058] The sulfated echinacea purpurea polysaccharide prepared in the above-mentioned embodiment 1 is applied to the immune regulation of chickens, and the use concentration is 2 -8 mg / mL.

[0059] Implementation 4

[0060] The sulfated echinacea purpurea polysaccharide prepared in the above-mentioned embodiment 1 is applied to the immune regulation of chickens, and the use concentration is 2 -9 mg / mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method and application of a sulfated echinacea purpurea polysaccharide. The preparation method comprises the following steps: S1, preparing a chlorosulfonic acid-pyridine esterification reagent; S2, preparing a uniform solution of echinacea purpurea polysaccharide from purified echinacea purpurea polysaccharide; S3, adding the esterification reagent into the uniform solution of the echinacea purpurea polysaccharide, and carrying out a sealed reaction under stirring at 80 DEG C for 3h; S4, after completing the reaction, carrying out cooling to the room temperature, regulating the pH value to 7, adding ethanol with the volume of 3-4 times and the concentration of 80%, and carrying out standing for settlement for 24h; and S5, adding water to dissolve thesediment, carrying out dialysis for 3d, concentrating a dialyzate, and carrying out freeze-drying to obtain the sulfated echinacea purpurea polysaccharide. The purified echinacea purpurea polysaccharide is subjected to sulfated modification to obtain immune-enhanced sulfated echinacea purpurea polysaccharide, and the sulfated echinacea purpurea polysaccharide is simple in preparation process, definite in component, controllable in quality, reliable in medicinal effect, suitable for large-scale production, safe in use and free of toxic or side effects, has a remarkable effect on regulating theimmune of dendritic cells of chicken and can be widely applied to an immune regulating medicine for birds and animals.

Description

technical field [0001] The invention belongs to the research field of traditional Chinese medicine extracts, and mainly relates to a preparation method and application of sulfated echinacea purpurea polysaccharides. Background technique [0002] The state of the body's immunity plays a decisive role in the disease resistance and productivity of the animal body. Dendritic cells are professional antigen-presenting cells in the animal body, and their state and quantity play a crucial role in the immune response in the animal body. Chinese herbal medicine has unique advantages, which can specifically or non-specifically enhance the body’s immunity. Years of research on polysaccharides have shown that polysaccharides from traditional Chinese medicine can enhance the immune function of the body, and have the advantages of safety, non-toxicity and stability of Chinese herbal medicine. The normal cells of the body have no toxic and side effects, and are good immune regulators of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/737A61P37/04
CPCA61K31/737A61P37/04C08B37/0003
Inventor 石达友白霖刘翠周珊珊谭盈陈晓丽郭世宁
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap