In-vitro anaerobic model device of oral biofilm and application of in-vitro anaerobic model device

Abstract

The invention relates to an in-vitro anaerobic model device of an oral biofilm and an application of the in-vitro anaerobic model device. The device comprises a nutrition supply and anaerobic generation system (1) and a biofilm culture system (2) which are in contact connection through frosted parts (10) and (11) on the systems, wherein a nutrient solution delivery inlet (3), a nutrient solution supply sieve (4) and an anaerobic bag storage chamber (9) are arranged on the nutrition supply and anaerobic generation system (1); a biofilm culture bin (6), a biofilm culture medium support (7), a nutrient solution outflow sieve (8) and a waste liquid discharge port (5) are arranged on the biofilm culture system (2). One to more biofilm culture media can be placed in the biofilm culture bin. Thedevice can effectively simulate the micro-ecological environment formed by a microbial biofilm in the oral cavity, facilitates replacement of the biofilm culture media, realizes multiple research needs and functional applications, and is easy to achieve, low in cost, simple and flexible to operate and beneficial to popularization and application in conventional laboratories.

Description

technical field [0001] The invention relates to an in vitro anaerobic model device of an oral cavity biofilm and an application thereof, belonging to the field of biofilm oral biomedicine. Background technique [0002] The human oral cavity has a complex anatomical structure and special physiological characteristics, and is colonized with a complex microbial community, which is one of the important micro-ecological environments of the human body (Journal of Clinical Microbiology, 2005, 43(11):5721-5732). Studies have shown that the composition and abundance of oral microbial communities are closely related to oral diseases such as caries, periodontal disease, and wisdom tooth pericoronitis, as well as systemic diseases such as pneumonia, tumors, cardiovascular diseases, and diabetes, which affect human health ( West China Journal of Stomatology, 2015, 33(6):555-560). The oral microbial community mostly performs its physiological functions in the form of "biofilms", and so...

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Problems solved by technology

The structure of artificial oral models established abroad is relatively complex, the equipment precision requirements are high, the cost is expensive, and the application cost is high, so the popularization and application are greatly restricted

The artificial oral models built by domestic laboratories are relatively simple in structure, easy to operate, and relatively low in application cost. The problems of these models are: (1) These artificial oral models are generally based on their own laboratory according to their own experimental needs. Existing conditions are established, for example, some are established for the uniformity of the film-forming medium to obtain repeatable results (CN105524869A), some are established for the roughness of the film-forming medium and multiple samples (CN106795472A), and some are established for continuous real-time monitoring (CN200952006Y; CN100408669C), used equipment such as anaerobic fermentation tanks and gas tanks to varying degrees, and was greatly limited due to the specificity of the equipment when conducting research on other functions and uses; (2) These devices include both dynamic models and Including the static model, the static model has certain limitations in simulating the oral environment; the anaerobic environment in the dynamic model is equipped with special anaerobic equipment, which is expensive; (3) In addition, the biofilm formation medium of these devices is relatively fixed , Once there are other research needs, it is often impossible to replace the medium

The above problems limit the popularization and application of these artificial oral models to varying degrees.

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