Method for polymerizing and breeding salt-tolerant colored foliage lagerstroemia indica based on gene detection
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A gene detection and salt-tolerant technology, applied in the field of crape myrtle breeding, can solve the problems such as the difficulty of planting flowering shrubs and leaf crape myrtle, and achieve the effect of overcoming the difficulty of identifying recessive traits, improving accuracy and reliability, and reducing backcross generations
Active Publication Date: 2018-10-16
NANTONG UNIVERSITY
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[0004] In order to overcome the defects existing in the existing technology, a method for breeding salt-tolerant Lagerstroemia indica based on gene dete
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[0028] The following describes the implementation of the present invention through specific specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.
[0029] Salt tolerance genes refer to genes that allow plants to express salt tolerance. Color-leaf genes refer to genes that make plant leaves express non-green colors.
[0030] In this example, the salt-tolerant Lagerstroemia spp. is a variety of Lagerstroemia in which both salt-tolerant genes and color-leaf genes are aggregated.
[0031] The invention provides a method for breeding salt-tolerant Lagerstroemia indica based on gene detection...
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Abstract
The invention provides a method for polymerizing and breeding salt-tolerant colored foliage lagerstroemia indica based on gene detection. The method comprises the following steps: hybridizing salt-sensitive colored foliage lagerstroemia indica and a salt-tolerant common lagerstroemia indica variety, so as to obtain an F1 generation plant group; stressing the F1 generation plant group through NaClsolution salt with different concentrations, and performing transcriptome sequencing on plant roots of the F1 generation plant group subjected to salt stress, so as to determine salt tolerance detection gene of lagerstroemia indica; performing transcriptome sequencing on leaves, with different colors during the same period, of the salt-sensitive colored foliage lagerstroemia indica, so as to obtain colored foliage detection gene of the lagerstroemia indica; detecting F1 generation plant to be detected according to the determined salt tolerance detection gene and the colored foliage detection gene, so as to breed and polymerize new lagerstroemia indica variety with salt tolerance detection gene. The method solves the problem that flowering shrub colored foliage lagerstroemia indica is difficult to plant in saline-alkali soil.
Description
Technical field [0001] The invention relates to the technical field of crape myrtle breeding, in particular to a method for breeding salt-tolerant crape myrtle leaves based on gene detection polymerization. Background technique [0002] Lagerstroemia, alias: itchy flower, itchy tree, purple flower, purple orchid, mosquito flower, western water bayberry, zinnia red, barkless tree, Latin name: Lagerstroemia indica L. Lythrumaceae, Lagerstroemia is a deciduous shrub or small Tree, up to 7 meters in height; smooth bark, gray or gray-brown; branches are twisted, slender branches, leaves alternate or sometimes opposite, papery, elliptical, broad rectangular or obovate, green to yellow when young , It is purple-black when mature or dry, and the back of the chamber is cracked; the seeds have wings and are about 8 mm long. Flowering from June to September, fruiting from September to December. The crape myrtle tree has a beautiful posture, smooth and clean trunks, and gorgeous flowers. I...
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