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A method to improve the correct recognition rate of maize haploid

A haploid and recognition rate technology, applied in the field of maize genetics and breeding, can solve the problems of long breeding cycle and high cost

Active Publication Date: 2021-06-08
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Breeding of excellent inbred lines is the core link of cultivating new varieties of maize. Using conventional methods for inbred line selection requires 6-8 generations of continuous selfing. The breeding cycle is long and the cost is high.

Method used

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  • A method to improve the correct recognition rate of maize haploid
  • A method to improve the correct recognition rate of maize haploid
  • A method to improve the correct recognition rate of maize haploid

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Experimental program
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Effect test

Embodiment 1

[0017] The experiment was carried out in the South Breeding Base of Henan Agricultural University in Jiusuo Town, Ledong County, Hainan in the winter of 2016. The high-oil inducible line CHOI2 was used as the male parent, and four different groups of hybrids Dan 598 / Zheng 22, Jing 24 / lx9801, Jun 9058 / Tie 7922, Qi 319 / Tai 8085) as the female parent, planted in a single-row area with a row spacing of 0.65 meters, a row length of 4 meters, and 20 plants in each row. In order to ensure that the flowering periods of the parents meet, the male parent induction line CHOI2 was planted in three phases, the first phase was sown at the same time as the female parent, the second phase was staggered five days later, and the third phase was staggered seven days later. Field management was performed routinely to ensure the consistency of cultivation conditions for each treatment. During the pollination period, CHOI2 was used as the male parent to induce artificial pollination on the above fe...

Embodiment 2

[0023] The experiment was carried out in the winter of 2016 at the Southern Propagation Base of Henan Agricultural University, Jiusuo Town, Ledong County, Hainan. The high-oil inducible line CHOI2 was used as the male parent, and four inbred lines of different groups (Tie C8605-2, E28, Zheng 22 and Jing 24) is the female parent; the planting method is a single-row area with a row spacing of 0.65 meters and a row length of 4 meters, with 20 plants in each row. In order to ensure that the flowering periods of the parents meet, the male parent induction line CHOI2 was planted in three phases, the first phase was sown at the same time as the female parent, the second phase was staggered five days later, and the third phase was staggered seven days later. Field management was performed routinely to ensure the consistency of cultivation conditions for each treatment. During the pollination period, CHOI2 was used as the male parent to induce artificial pollination on the above female...

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Abstract

The invention discloses a method for improving the correct recognition rate of corn haploids. The corn haploids are sorted based on the double threshold value of oil content in the grains. The corn grains induced by the high-oil inducing line include haploid grains, normal hybrid two For ploidy grains and embryo-aborted grains, the upper threshold and lower threshold are set according to the oil content of different types of grains. Grains with oil content less than the upper threshold and greater than the lower threshold are haploid grains, which are sorted by nuclear magnetic resonance The system sorts the maize kernels induced by the high-oil inducing line to obtain haploid kernels. The present invention can accurately distinguish haploid, embryo-aborted grains, and normal diploid grains by setting double thresholds of oil content, effectively improving the accuracy of haploid selection, reducing its misselection rate and artificial The cost of secondary sorting has further promoted the engineering process of haploid breeding.

Description

technical field [0001] The invention belongs to the technical field of corn genetics and breeding, and in particular relates to a method for improving the correct recognition rate of corn haploid. Background technique [0002] Breeding of superior inbred lines is the core link of cultivating new varieties of maize. Using conventional methods for inbred line selection requires 6-8 generations of continuous selfing, which has a long breeding cycle and high cost. With the continuous development of breeding technology, maize haploid breeding technology only needs two generations a year to obtain pure lines, which significantly shortens the breeding cycle and improves breeding efficiency (Wei Junjie, 2011). Favored by maize breeders, it has become one of the three core maize breeding technologies comparable to molecular marker-assisted breeding technology and transgenic technology (Chen Shaojiang, 2012). High-efficiency production of haploids is a prerequisite for the use of thi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 李浩川刘宗华曲彦志汤继华付志远丁冬李卫华薛亚东郭战勇
Owner HENAN AGRICULTURAL UNIVERSITY
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