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A culture medium composition containing fermentation waste liquid and its preparation method and fermentation method

A technology for fermentation waste liquid and culture medium, which is applied in the field of re-fermentation and utilization of fermentation waste liquid, can solve problems such as hindering the healthy development of enterprises, high daily operating costs, and large one-time investment.

Active Publication Date: 2022-05-03
杭州雪域生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no mature complete set of technology applied to production practice at home and abroad.
The main problem is that the one-time investment is too large, or the daily operation cost is too high, most manufacturers cannot bear it, and have to maintain the status quo of excessive emissions for a long time
[0007] With the development of industry and the expansion of production scale, the output of fermentation waste liquid will also continue to increase. If it cannot be effectively and properly treated, it will increase the economic and environmental burden of the enterprise and hinder the healthy development of the enterprise.

Method used

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  • A culture medium composition containing fermentation waste liquid and its preparation method and fermentation method
  • A culture medium composition containing fermentation waste liquid and its preparation method and fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 0.06 g of iron-containing yeast and 30 g of superfine corn powder were added to 1 L of Agaricus blazei mycelium liquid fermentation waste liquid, sterilized at 121° C. for 30 minutes, and then cooled.

[0030] Inoculate Agaricus blazei seeds into the above-mentioned liquid medium, carry out liquid fermentation, temperature 25°C, aeration and stirring, fermentation time 15d, centrifuge the cultured fermentation liquid at 2000rpm, collect mycelia, wash and dry to obtain iron-containing Agaricus blazei mycelium.

Embodiment 2

[0032] 0.18 g of iron-containing yeast and 10 g of superfine corn powder were added to 1L of Agaricus blazei mycelium liquid fermentation waste liquid, sterilized at 121° C. for 15 minutes, and then cooled.

[0033] Inoculate Agaricus blazei seeds into the above-mentioned liquid medium, carry out liquid fermentation, temperature 27°C, aeration and stirring, fermentation time 3d, centrifuge the cultured fermentation liquid at 10000rpm, collect mycelia, wash and dry to obtain iron-containing Agaricus blazei mycelium.

Embodiment 3

[0035] 0.09 g of iron-containing yeast and 25 g of superfine corn powder were added to 1 L of Agaricus blazei mycelium liquid fermentation waste liquid, sterilized at 121° C. for 20 minutes, and then cooled.

[0036] Inoculate Agaricus blazei seeds into the above-mentioned liquid medium, carry out liquid fermentation, the temperature is 25°C, aeration and stirring, the fermentation time is 5d, the cultured fermentation liquid is subjected to plate and frame pressure filtration with a pressure of 60kg, the mycelium is collected, and after The iron-containing Agaricus blazei mycelium is obtained by washing and drying.

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Abstract

The invention provides a culture medium composition containing fermentation waste liquid, a preparation method and a fermentation method thereof, and belongs to the technical field of fermentation waste liquid re-fermentation and utilization. The mycelium dry weight and iron content of Agaricus blazei obtained by liquid fermentation using the culture medium containing fermentation waste liquid of the present invention are significantly improved compared with the fermentation waste liquid and the mixing ratio of fermentation waste liquid and fermentation stock solution. , and also has obvious advantages in terms of cost, turning waste into treasure, and environmental friendliness.

Description

technical field [0001] The invention relates to the technical field of re-fermentation and utilization of fermentation waste liquid, in particular to a culture medium composition containing fermentation waste liquid, a preparation method and a fermentation method thereof. Background technique [0002] With the progress of human civilization and the continuous development of the national economy, people pay more and more attention to their own health. [0003] Agaricus blazei, also known as Brazil mushroom or Brazil mushroom, is native to Brazil, Peru and other places, and belongs to the category of edible and medicinal mushrooms. Agaricus blazei is extremely nutritious, has an excellent taste, pure and delicious taste, and has high edible value. The protein composition includes 18 kinds of amino acids, the 8 kinds of essential amino acids for the human body are complete, and it also contains a variety of vitamins and ergosterol. In Japan, it is one of the treasures that th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 徐深录李肖娟
Owner 杭州雪域生物技术有限公司
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