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Secondary antibody staining method for fully-automatic immunohistochemical staining apparatus

A technology of immunohistochemistry and staining methods, applied in the field of secondary antibody staining, can solve the problems of inability to use an automatic immunohistochemical staining instrument and low staining sensitivity, reduce non-specific staining background, improve staining intensity, and satisfy pathological diagnosis. desired effect

Inactive Publication Date: 2018-10-19
杭州依美洛克医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, general immunohistochemical reagents are only suitable for manual staining requirements, and cannot be applied to automatic immunohistochemical staining machines, resulting in low staining sensitivity

Method used

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  • Secondary antibody staining method for fully-automatic immunohistochemical staining apparatus

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Embodiment 1

[0040] A secondary antibody staining method used in an automatic immunohistochemical staining apparatus, including the following methods:

[0041] S1. Add 150ul of blocking solution to the tissue section to inactivate the endogenous peroxidase in the cells of the tissue section;

[0042] S2. Add 150ul of the corresponding primary antibody to the tissue section to react with the antigen protein in the tissue section cell;

[0043] S3. Add 150ul of reaction enhancement solution to the tissue section;

[0044] S4. Add 150ul of polymer secondary antibody to the tissue section to specifically bind to the primary antibody;

[0045] S5. Add a mixture of 7.5ul DAB concentrate and 150ul DAB buffer to the tissue section, and the DAB precipitate will be colored;

[0046] S6. Add 150ul of hematoxylin counterstain solution to the tissue section to combine with chromatin to show blue.

[0047] Another solution, a secondary antibody staining method for a fully automated immunohistochemical staining appa...

Embodiment 2

[0070] As shown in Example 1, the only difference is that the blocking liquid is a hydrogen peroxide solution, and the concentration of the hydrogen peroxide solution is 3%.

Embodiment 3

[0072] As shown in Example 1, the only difference is that the blocking liquid is a hydrogen peroxide solution, and the concentration of the hydrogen peroxide solution is 3.5%.

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Abstract

The invention relates to a secondary antibody staining method, in particular to a secondary antibody staining method for a fully-automatic immunohistochemical staining apparatus. The secondary antibody staining method comprises the following steps of S1, adding a blocking fluid into a tissue slice, and deactivating endogenous peroxidase in cells of the tissue slice; S2, adding a corresponding primary antibody into the tissue slice, and reacting and combining with antigen protein in the cells of the tissue slice; S3, adding a reaction reinforcing solution into the tissue slice; S4, adding a polymer secondary antibody into the tissue slice, and specifically combining with the primary antibody; S5, adding a mixed solution of DAB (diaminobenzidine) concentration solution and DAB buffer solution into the tissue slice, and precipitating and staining DAB; S6, adding a hematoxylin redyeing solution into the tissue slice, combining with chromatin, and displaying blue color. The secondary antibody staining method has the advantages that the required reagent components in the conventional immunohistochemistry experiment are optimized, the staining intensity of immunohistochemistry is improved, the possibility of production of non-specific staining background is reduced, and the staining quality is greatly improved.

Description

Technical field [0001] The invention relates to a secondary antibody staining method, in particular to a secondary antibody staining method used in an automatic immunohistochemical staining instrument. Background technique [0002] In clinical pathological diagnosis and morphological research, immunohistochemistry (immunohistochemistry for short) staining is a very important technique and method. Immunohistochemistry technology began to be used in pathological diagnosis in the 1970s, and has been widely used in the global pathology community. It has become an indispensable part of the routine work of pathologists. Immunohistochemistry technology has the advantages of specificity, strong sensitivity and easy operation, which makes immunohistochemistry technology widely promoted and applied in the field of disease diagnosis, especially clinical pathological diagnosis and tumor transformation diagnosis. Immunohistochemistry technology can not only improve the accuracy of pathologic...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N33/532
CPCG01N1/30G01N33/532
Inventor 袁淦英李思勇余向东
Owner 杭州依美洛克医学科技有限公司
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