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Method for measuring and correcting hematocrit (HCT) in electrochemical biosensor

A hematocrit and biosensor technology, which is applied in the field of hematocrit measurement and correction in electrochemical biosensors, can solve the problems of measurement current reduction, measurement current increase, and test result interference, so as to eliminate influence and test accurately , the effect of improving the accuracy of the results

Inactive Publication Date: 2018-10-19
北京乐普诊断科技股份有限公司
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Problems solved by technology

However, these handheld electrochemical biosensors are easily affected by blood hematocrit when testing blood samples, which can interfere with the test results
Taking blood sugar measurement by current method as an example, when the blood hematocrit is too low, the measurement current may increase, resulting in a high blood sugar value; on the contrary, when the blood hematocrit is too high, the measurement current may be reduced, It leads to low blood sugar value, and the large deviation can even reach more than 50%. Therefore, eliminating the influence of hematocrit is one of the keys to improve the accuracy of this type of product

Method used

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  • Method for measuring and correcting hematocrit (HCT) in electrochemical biosensor
  • Method for measuring and correcting hematocrit (HCT) in electrochemical biosensor
  • Method for measuring and correcting hematocrit (HCT) in electrochemical biosensor

Examples

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Embodiment example 1

[0057] Embodiment Example 1: determination of blood hematocrit measurement equation

[0058] Step 1: Prepare multiple blood samples, and adjust the hematocrit to 20%, 30%, 40%, 50%, 60%, and 70% respectively. Test the resistance value of blood samples with different hematocrits with a glucose sensor. As shown in Table 1:

[0059] HCT%

R(KΩ)

20

11.044

30

14.933

40

20.722

50

30.311

60

43.100

70

62.015

[0060] Table 1

[0061] Step 2: Utilize the data in Table 1, take the resistance value as the X axis, and take the blood hematocrit as the Y axis, and perform linear fitting to obtain the relationship function between blood (HCT%) and resistance value (R), namely

[0062] HCT%=-0.0164*R 2 +2.1212*R+0.7997(Eq.1)

[0063] Substituting the measured resistance value (R) into the above Eq.1 equation, the calculated blood hematocrit is obtained, and the correlation with the measured standard blood hematocrit is ...

Embodiment example 2

[0065] Embodiment Example 2: Final blood glucose concentration calibration equation determination

[0066] Step 1: Prepare multiple blood samples, adjust the hematocrit to 20%, 30%, 40%, 50%, 60%, and 70%, respectively, and the blood sugar concentration is 600mg / dL, and calibrate with the YSI2300 glucose-lactic acid analyzer, that is C corr = 600 mg / dL. Blood glucose concentration was measured using a glucose sensor without a hematocrit correction equation (C mea ). As shown in table 2:

[0067]

[0068]

[0069] Table 2

[0070] Step 2: Statistically analyze the data in step 1 to obtain the correction equation for the final blood glucose concentration:

[0071] C corr =C mea / (-0.0152*HCT%+1.6082) (Eq.2)

[0072] Step 3: Program the blood glucose concentration correction equation (Eq. 2) in step 2 into the software used with the electrochemical biosensor or on the calibration chip of the electrochemical biosensor.

[0073] Figure 5 It is the comparison of blo...

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Abstract

The invention provides a method for measuring and correcting hematocrit (HCT) in an electrochemical biosensor. According to the method, analyte content to be determined is corrected by utilizing a determined HCT value, so as to eliminate influences, caused by the HCT, on the determination of the analyte content. The method comprises the steps of measuring the HCT and correcting analyte concentration. The method for measuring the HCT comprises the following steps: firstly, acquiring resistance value (R) of different HCT blood samples; manufacturing a relativity curve between the HCT and the blood resistance (R); then determining HCT value (HCT percent) of the blood samples according to the pre-determined relativity curve between the resistance and the HCT. The method for correcting the analyte concentration comprises the following steps: establishing a correction equation; measuring the analyte concentration and calculating final concentration value of analyte (Ccorr) by utilizing the measured HCT value (HCT percent) and measured analyte concentration value (Cmea).

Description

technical field [0001] The invention relates to the technical field of biological measurement, in particular to a method for measuring and correcting hematocrit in an electrochemical biosensor. Background technique [0002] Electrochemical biosensors react with biomolecules (antigens, antibodies, metabolites, etc.) technology. Widely used in environmental, medical and other fields, its main features are good selectivity and high sensitivity. Among them, hand-held electrochemical biosensors are widely used in bedside detection and household chronic disease monitoring due to their simple structure, easy operation, and portability, such as hand-held blood lipid testers and blood glucose meters. However, these handheld electrochemical biosensors are easily affected by blood hematocrit when testing blood samples, thereby causing interference to the test results. Taking blood sugar measurement by current method as an example, when the blood hematocrit is too low, the measuremen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N15/05
CPCG01N27/26G01N15/05
Inventor 郭佳范树芹邱笑违董飒英
Owner 北京乐普诊断科技股份有限公司
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