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Method of separating lactotransferrin from whey liquid

A technology of lactoferrin and whey, applied in the field of obtaining lactoferrin, can solve the problem of high liquid cost and achieve the effects of reduced investment, easy management, fast and complete production process

Inactive Publication Date: 2002-10-23
徐跃
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very costly to directly process such a large amount of liquid with chromatographic separation technology.

Method used

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  • Method of separating lactotransferrin from whey liquid
  • Method of separating lactotransferrin from whey liquid
  • Method of separating lactotransferrin from whey liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Isolation and preparation of lactoferrin from whey

[0044] Cheddar cheese whey from cheese factories is first skimmed in a centrifuge. The content of lactoferrin in skim cheddar cheese whey is 93 mg / L. Add food grade CaCl to 420 liters of skim cheddar whey 2 3.4 kg and NaH 2 PO 4 2H 2 O 0.62 kilograms, then adjust the pH value to 7.5 with 6 equivalents of sodium hydroxide while stirring. After stirring for 5 minutes, let stand for 10 minutes. The suspension is separated with a liquid-solid centrifuge (or a plate and frame filter press, or a drum vacuum filter or other filtering machinery) to obtain 38.2 kg of calcium phosphate sediment. The clarified liquid is sent to the whey protein and lactose production workshop. The precipitated mud is freeze-dried (or low-temperature vacuum-dried, or spray-dried after adding 80 kg of water) to obtain 8.4 kg of calcium phosphate precipitated powder (identifications such as its electrophoresis analysis are shown i...

Embodiment 2

[0045] Example 2 Isolation and preparation of lactoferrin from whey

[0046] The content of lactoferrin in raw cheese whey was 124.9 mg / L. Adding CaCl to 540 liters of raw cheese whey 2 4.5 kg and NaH 2 PO 4 2H 2 O 0.8 kg, then adjust the pH value to 6.5 with 6 equivalents of sodium hydroxide while stirring. After stirring for 5 minutes, let stand for 10 minutes. The suspension is separated with a liquid-solid centrifuge (or a plate and frame filter press, or a drum vacuum filter or other filtering machinery) to obtain 15 kg of calcium phosphate sediment. The clarified liquid is sent to the whey protein and lactose production workshop. The precipitated mud is freeze-dried (or vacuum-dried at low temperature, or spray-dried after adding 40 kg of water) to obtain 3.4 kg of calcium phosphate precipitated powder, which is stored at low temperature (-10°C) for future use. Each kilogram of precipitation powder contains 14.5 grams of lactoferrin, and 73% of the lactoferrin in...

Embodiment 3

[0047] Example 3 Preparation of High Purity Lactoferrin

[0048] Equilibrate a carboxymethyl Sephadex resin cation exchange column (or a commercial carboxymethyl cation exchange column, Carboxymethyl-Productive TM ). 30 milliliters of concentrated solutions (made by Example 1) are pumped in from the top of the column, and then gradient eluted with 0.7 mol / liter of sodium chloride acetate buffer (pH3.8), and the flow rate of the eluent is 2 milliliters / liter minute. Collect liquid from 120 to 170 milliliters, total 50 milliliters, the separation yield of lactoferrin column is over 90%. The collected liquid was deionized with a dialysis membrane and then freeze-dried. The lactoferrin purity of the product is 95%, and the total yield is 61.9%.

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Abstract

The method for separating lactoferritransfer protein from whey liquor includes: 1. adopting noe-step calcium phosphate affinity precipitation method to selectively preprecipitate and separate micro amount of lactoferritransfer protein from whey liquor to obtain its precipitate powder; 2. extracting said precipitate powder by using buffer solution or salt water to obtain extract containing lactoferritransfer protein; 3. separating said extract liquor by using ultrafiltration membrane whose molecular weight trapping value is 5000 daltons, concentrating, spraying or freeze-drying to obtain its powder product whose content is 40-50%; 4. also can use cation exchange column to further purify the concentrate, the spraying or freeze-drying so as to obtain the lactoferritransfer protein powder product whose content is 90-95%.

Description

technical field [0001] The present invention relates to a method for obtaining lactoferrin from animals, especially a method for isolating lactoferrin from whey. Background technique [0002] Transferrins are a group of glycoproteins with similar structures and properties. Its similarity includes that the high-level structure of all transferrins consists of a single-chain polypeptide forming two similar but non-uniform iron-binding sites (one molecule of protein binds two molecules of Fe 3+ ) and contain polysaccharides (Glycan) and their molecular weights are approximately in the range of 76000-81000 Daltons. Transferrin is widely distributed in physiological fluids and cells of vertebrates, such as milk, saliva, bile, pancreatic juice, tears and plasma. [0003] Serum transferrin (Serum Transferrin), ovalbumin transferrin (Ovatransferrin) and lactoferrin (Lactoferrin) are the main members of the transferrin family. Transferrin was originally named for its transport and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K14/79
Inventor 徐跃
Owner 徐跃
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