Method for determining window periods for provenance removal of over-wintering cyanobacteria in shallow lakes

Abstract

The invention provides a method for determining window periods for the provenance removal of overwintering cyanobacteria in shallow lakes. The method is characterized in that the adopted cyanobacteriahas three key functional genes in the resuscitation process; the expressions of a phycocyanin synthesis gene (PC-IGS), a pseudo-empty cell synthesis gene (gvpC) and a cyanotoxin synthesis gene (mcyA)directly relate to activity recovery and floating of the cyanobacteria, so that the window periods capable of accurately reflecting the provenance removal of the overwintering cyanobacteria are established; the expression quantities of the three key genes in cells in the overwintering resuscitation of the cyanobacteria are determined by adopting a fluorescent quantitative PCR technology, and theresuscitation process of the water-blooming cyanobacteria with metabolic activity is determined, so that the window periods for the provenance removal of the cyanobacteria in different lake areas aredetermined. The method provided by the invention has the advantages that three key parameter indexes for reflecting the resuscitation of the overwintering cyanobacteria are integrated, the optimum window periods for the provenance removal of the cyanobacteria in the different lake areas are discriminated, the method is more accurate than a traditional method for determining the algae standing stock, is more time and labor-saving than a pressure capillary method for studying the morphologies and the characteristics of pseudo-empty cells through separating and purifying the pseudo-empty cells from the cells and other methods, and provides a technical support for the water-blooming control of the cyanobacteria in the lakes.

Description

technical field [0001] The invention belongs to the field of water environment treatment and cyanobacteria bloom control, and relates to eutrophication treatment and ecological restoration technology of large shallow lakes and reservoirs, and in particular to a method for determining the window period for clearing the provenance of overwintering cyanobacteria in shallow lakes. Background technique [0002] With the rapid development of my country's social economy, the problem of eutrophication of lakes is becoming more and more serious, which has seriously affected the development of society and the drinking water problem in some areas. According to the Bulletin on the State of the Environment in China in 2013, the proportions of eutrophic, mesotrophic and oligotrophic lakes (reservoirs) are 27.8%, 57.4% and 14.8%, respectively. When phosphorus and nitrogen in lake water increase, it can lead to excessive reproduction of algae and other aquatic plants, causing "algal blooms"...

AI Technical Summary

Problems solved by technology

However, this invention does not explain how to determine the method for the recovery period of cyanobacteria in lakes, so that engineering measures to remove and control recovery algae cannot be carried out accurately, and the effect may be unsatisfactory due to inaccurate implementation time

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