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Composite microbial agent and preparation method thereof and application in high-salt soil restoration

A compound microbial inoculant and compound microorganism technology, which is applied in the field of soil remediation, can solve the problems of large influence on microbial activity, and achieve the effect of reducing the content of organic pollutants and having a good application prospect.

Active Publication Date: 2018-10-26
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, people have done a lot of research on soil remediation by microorganisms. However, there are few researches on soil remediation under relatively extreme conditions, such as high salinity conditions. This is mainly because extreme conditions have a greater impact on the activity of microorganisms.

Method used

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  • Composite microbial agent and preparation method thereof and application in high-salt soil restoration
  • Composite microbial agent and preparation method thereof and application in high-salt soil restoration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The composite microbial agent is composed of Hyphomicrobiumdenitrificans FJNU-R8 agent and Arthrobacter sp. YC-RL1 agent with a mass ratio of 1:1.2, wherein the denitrificans ( The live bacteria content of Hyphomicrobium denitrificans) FJNU-R8 is 5×10 9 ~1.0×10 10 cfu / mL, the live bacteria content of Arthrobacter sp. YC-RL1 is 5×10 9 ~1.0×10 10 cfu / mL. The preparation method of the composite microbial bacterial agent comprises:

[0022] (1) Preparation of Hyphomicrobium denitrificans FJNU-R8 bacterial agent:

[0023] Hyphomicrobium denitrificans FJNU-R8 was cultured in seed medium at 30°C for 60 hours, then inoculated into fermentation medium at 5% by volume and cultured at 30°C for 5 days.

[0024] The seed medium consisted of the following components: 6 g / L methanol, 2.5 g / L ammonium sulfate, 1.4 g / L monopotassium phosphate, 3 g / L disodium hydrogen phosphate, 1 g / L magnesium sulfate and water , the pH of the seed medium was 7.0.

[0025] The content of each sub...

Embodiment 2

[0030] The composite microbial agent is composed of Hyphomicrobiumdenitrificans FJNU-R8 agent and Arthrobacter sp. YC-RL1 agent with a mass ratio of 1:1.5, wherein the denitrificans ( The live bacteria content of Hyphomicrobium denitrificans) FJNU-R8 is 5×10 9 ~1×10 10 cfu / mL, the live bacteria content of Arthrobacter sp. YC-RL1 is 5×10 9~1×10 10 cfu / mL. The preparation method of the composite microbial bacterial agent comprises:

[0031] (1) Preparation of Hyphomicrobium denitrificans FJNU-R8 bacterial agent:

[0032] Hyphomicrobium denitrificans FJNU-R8 was cultured in seed medium at 32°C for 48 hours, then inoculated into fermentation medium at 5% by volume and cultured at 28°C for 6 days.

[0033] The seed medium consisted of the following components: methanol at 5 g / L, ammonium sulfate at 4 g / L, potassium dihydrogen phosphate at 0.8 g / L, disodium hydrogen phosphate at 5 g / L, magnesium sulfate at 2 g / L, and water, The pH of the seed medium was 6.8.

[0034] The cont...

Embodiment 3

[0039] The composite microbial agent is composed of Hyphomicrobiumdenitrificans FJNU-R8 agent and Arthrobacter sp. YC-RL1 agent with a mass ratio of 1:1.3, wherein the denitrificans ( The live bacteria content of Hyphomicrobium denitrificans) FJNU-R8 is 5×10 9 ~1×10 10 cfu / mL, the live bacteria content of Arthrobacter sp. YC-RL1 is 5×10 9 ~1×10 10 cfu / mL. The preparation method of the composite microbial bacterial agent comprises:

[0040] (1) Preparation of Hyphomicrobium denitrificans FJNU-R8 microbial agent:

[0041] Hyphomicrobium denitrificans FJNU-R8 was cultured in seed medium at 28°C for 72 hours, then inoculated into fermentation medium at 3% by volume and cultured at 25°C for 6 days.

[0042] The seed medium consisted of the following components: methanol at 10 g / L, ammonium sulfate at 2 g / L, potassium dihydrogen phosphate at 2.4 g / L, disodium hydrogen phosphate at 6 g / L, magnesium sulfate at 3 g / L, and water, The pH of the seed medium was 7.0.

[0043] The co...

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Abstract

The invention discloses a composite microbial agent. The composite microbial agent comprises hyphomicrobium denitrificans FJNU-R8 microbial agent and arthrobacter sp.YC-RL1 microbial agent. The invention also discloses a preparation method of the composite microbial agent and an application in high-salt soil restoration. According to the preparation method, the hyphomicrobium denitrificans FJNU-R8microbial agent and the arthrobacter sp.YC-RL1 microbial agent are used for restoring the high-salt soil for the first time, and the content of organic pollutants in the high-salt soil can be remarkably reduced by combining the two bacterial agents, so that the composite microbial agent has a good application prospect.

Description

[0001] Technical solutions [0002] The invention belongs to the technical field of soil remediation, and in particular relates to a composite microbial bacterial agent, a preparation method thereof and an application in high-salt soil remediation. Background technique [0003] Soil is a resource that human beings depend on for survival and an important place for material and energy cycles in the ecosystem. Soil environment and ecological security are important guarantees for sustainable development. With the rapid development of the economy, the production and domestic wastewater, waste gas, waste residue and other wastes that have not been effectively treated have increased sharply. Many harmful substances have continuously entered the soil and accumulated. The overall situation of soil pollution is severe. Agricultural security and sustainable development have a greater impact. [0004] In recent years, there have been many studies on soil remediation by microorganisms. H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C09K17/40C09K101/00
CPCB09C1/105B09C2101/00C09K17/40C09K2101/00
Inventor 谢悦波陈求稳何庆成金波姚竣耀
Owner 江苏世邦生物工程科技有限公司