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SNP markers related to ear length traits of millet and their detection primers and applications

A technology for millet and ear length is applied in the field of SNP markers and their detection primers, which can solve the problems of unreported research on the development and application of SNP molecular markers.

Active Publication Date: 2021-12-21
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the QTL mapping and SNP markers related to the quantitative traits of millet is mainly focused on the development and utilization of SSR markers, but the development and application of SNP molecular markers using degenerate genome sequencing of population inbred lines to detect polymorphisms has not yet been reported.

Method used

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  • SNP markers related to ear length traits of millet and their detection primers and applications
  • SNP markers related to ear length traits of millet and their detection primers and applications
  • SNP markers related to ear length traits of millet and their detection primers and applications

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 High-throughput sequencing and SNP marker information analysis

[0034] In this example, the materials of the male parent "Ji Zhanggu No. 1" and the female parent "A2" of millet, the F1 generation materials of the two hybrids, and 441 materials of the F2 population of the 13th generation of selfing, that is, recombinant inbred lines (Recombinant inbred lines , abbreviated as RILs). Each material was planted in Sanya, Hainan, and the trait data such as ear length were recorded and sorted. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the data of ear length traits, the associated SNP markers were obtained, and the SNP markers were verified by clonal sequencing.

[0035] In this example, the RADseq method was used to extract the genomic DNA of each sample individual, a total of 444 samples, including 441 RILs, 2 parents, and 1 F1 individual. The qual...

Embodiment 2

[0058] Embodiment 2 SNP marker verification

[0059] According to the predicted bin marker and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select the amplified products with normal primer amplification and the compounded predicted size of the PCR amplification products for gel cutting recovery and sequencing, and select the labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials. Using the F1 and F2 generation materials as templates, carry out PCR amplification of SNP marker primers and sequencing of the results, and select marker primers that are consistent with genotyping and phenotypic traits.

[0060] First, according to the sequencing results after PCR product recovery, the markers for the di...

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Abstract

The application discloses a SNP marker related to the ear length trait of millet, detection primers and application thereof. The SNP marker related to the trait of ear length of millet in the present application is located in the bin marker from the 30390189bp to the 30551864bp of the first chromosome, and the SNP marker is closely linked with the ear length of millet. The SNP markers of this application related to the traits of ear length of millet can be used for molecular marker-assisted breeding of traits of ear length of millet. The detection of the SNP markers can predict the ear length of millet, which provides a basis for early identification or screening breeding of ear length traits of millet. The scientific basis has important theoretical and practical guiding significance for accelerating the genetic selection or improvement of millet varieties. The primer pair used in this application to detect SNP markers can be used to type the ear length of millet and detect differences in SNP expression.

Description

technical field [0001] The application relates to the technical field of millet breeding, in particular to a SNP marker related to the trait of ear length of millet, detection primers and applications thereof. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] Spike length and plant height are important factors affecting millet yield. It is of great significance to study the genes related to the control of yield traits such as ear length and their position functions in guiding millet genetics and breeding. [0004] Single nucleotide polymorphism (singlenucleotide polymorphism, SNP) is a DNA sequence polymorphism caused by the variation of a single nucleotide base at the genome level. It has a large number in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵治海魏玮冯小磊李欣儒张耕耘范光宇张雅莉邱风仓张丽娜
Owner ZHANGJIAKOU ACAD OF AGRI SCI