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Freshwater fish compound preservative as well as preparation method and application thereof

A technology for composite preservation of freshwater fish, applied in biochemical equipment and methods, bacteria used in food preparation, applications, etc., can solve the problem of high residual amount of chemical preservatives, achieve enhanced antiseptic effect, wide antibacterial spectrum, and extend shelf life period effect

Inactive Publication Date: 2018-10-30
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of high residual amount of chemical preservatives in aquatic products, and to provide a freshwater fish composite preservative based on safe, non-toxic, efficient and broad-spectrum antimicrobial peptides and its preparation method and application

Method used

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  • Freshwater fish compound preservative as well as preparation method and application thereof
  • Freshwater fish compound preservative as well as preparation method and application thereof
  • Freshwater fish compound preservative as well as preparation method and application thereof

Examples

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Effect test

example 1

[0030] Example 1 is a blank reference, is a sample of sterile water treatment, example 2 is the treatment group of antibacterial lipopeptide crude extract, and example 3, 4 and 5 are antibacterial lipopeptide crude extract, bacteriocin crude extract and thymol Treatment group for a mixture of three preservatives.

Embodiment 1

[0031] Embodiment 1 (blank reference)

[0032] Treatment A: Knock the head of fresh and live crucian carp to death, remove the head, tail and viscera, rinse with sterile water for 15-20 seconds, and drain in an environment below 10°C;

[0033] Treatment B: Grass carp slices, cut into 20g / piece, length 9cm, width 4cm, thickness 0.5cm grass carp fillets, washed with sterile water for 15-20S, and drained in an environment below 10°C.

[0034] Place the above-mentioned pretreated freshwater fish body in sterile water, and immerse all the fish body in the sterile water for 15 minutes. Drain the soaked fish in an environment below 10°C, place them in sterile trays, and store them at 4°C.

Embodiment 2

[0035] Embodiment 2 (contrast)

[0036] Preparation of antimicrobial lipopeptide crude extract: Bacillus amyloliquefaciens ES-2-4 was inoculated on test tube slant PAD agar medium (potato (peeled) 200g, glucose 20g, agar 15-20g, distilled water 1000ml, natural pH), 37°C Cultivate the activated strains in the incubator for 24 hours, and then inoculate the activated strains in the BPY medium containing the seed medium (5g beef extract, 5g yeast extract, 5g sodium chloride, 10g fish powder peptone, 10g glucose, 1000mL water. In a Erlenmeyer flask sterilized at 115° C. for 20 minutes), culture at 37° C. and 180 rpm for 24 hours to make a seed solution. The seed liquid was inoculated in Landy fermentation medium (glucose 42.0g, sodium glutamate 14.0g, MgSO 4 ·7H 2 O0.5g, KCl 0.5g, KH 2 PO 4 1g, FeSO 4 ·7H 2 O 0.15mg, MnSO 4 ·7H 2 O 5mg, CuSO 4 ·5H 2O 0.16mg, water 1000mL. 115°C autoclave for 20min), ferment at 33°C and 180rpm for 36h, take out, centrifuge at 9000rpm for...

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Abstract

The invention relates to a freshwater fish compound preservative and a freshness retaining method. The compound preservative is prepared from bacteriocin crude extract, an antimicrobial lipopeptide crude extract, thyme camphor and water. The freshwater fish compound preservative is an environment-friendly compound preservative prepared for main putrefying flora in freshwater fish, has the characteristics of nontoxicity, a wide antimicrobial spectrum, high antibacterial property and is applicable to preservation and antisepsis of the freshwater fish; the use method is simple, and the productioncost is low; the shelf life of the freshwater fish can be effectively prolonged to 9 d, so that the quality and the storage stability of refrigerated fish are improved, and the freshwater fish compound preservative has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of fresh-keeping of aquatic products, and relates to a composite fresh-water fish fresh-keeping agent, a preparation method thereof and an application in fresh-water fish fresh-keeping. Background technique [0002] Freshwater fish has high nutritional value, which is rich in calcium, iron, vitamins, essential amino acids, polyunsaturated fatty acids and other nutrients, and is deeply loved by consumers. However, due to the high protein and water content in freshwater fish, it contains a variety of Unsaturated fatty acids and endogenous enzymes make freshwater fish easy to corrupt, and the corruption rate of freshwater fish in my country is as high as 90%. The spoilage of freshwater fish is mainly affected by microorganisms from itself and the environment, as well as its own enzymes and fat oxidation, among which microorganisms are the main factor causing spoilage. Therefore, studying the growth of fish spoi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B4/20A23B4/22C12P21/00C12R1/07C12R1/25
CPCA23B4/20A23B4/22C07K14/32C07K14/335A23V2002/00A23V2400/169A23V2200/10A23V2250/206A23V2250/55A23V2250/30
Inventor 吕凤霞陆兆新李秀秀曾维伟别小妹赵海珍张充
Owner NANJING AGRICULTURAL UNIVERSITY
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