Rapid propagation method for tissue culture of pinellia ternata

A technology of tissue culture and Pinellia, applied in the field of plant cell engineering, to achieve the effects of increasing the yield of Pinellia, shortening the multiplication time, accelerating the industrial production of Pinellia and high-quality development

Inactive Publication Date: 2018-11-06
甘肃源宜生物科技有限公司
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Problems solved by technology

But at present there is no high-quality rapid propagation method of pinellia tissue culture

Method used

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Embodiment Construction

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present invention belong to the protection scope of the present invention.

[0021] The rapid propagation method of pinellia tissue culture is as follows:

[0022] (1) Disinfection and inoculation of pinellia explants: Take 5-8cm tall, well-growth, and tender pinellia petioles as explants, wash off the dust on the surface, wash with detergent for 5 minutes, and then put them under running water. Rinse for 2 hours, and finally rinse with sterile water and place on an ultra-clean workbench. Under sterile conditions, first put the petiole in 0.1% mercury liter solution, shake gently for 7 minutes, and then rinse the petiole with sterile water ...

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Abstract

The invention discloses a rapid propagation method for tissue culture of pinellia ternata. The method comprises the following steps: petiole of pinellia ternata is taken as an explant, cleaned and sterilized; the petiole of pinellia ternata is cut into proper size and inoculated on an induction medium to induce callus; differentiated callus of pinellia ternata is transferred to a proliferation medium to differentiate pinellia ternata seedlings; the differentiated pinellia ternata seedlings are inoculated into a sugar-free rapid propagation culture container, CO2 is introduced in 3-5 days, andwell-grown and rooted pinellia ternata seedlings are obtained and can be transplanted. The rapid propagation method for tissue culture of pinellia ternata is superior to traditional methods such as tubers, plant buds and the like for asexual propagation, and the problems of slow propagation of the seedlings, small quantity, virus carrying in the seedlings and the like are solved.

Description

technical field [0001] The invention relates to the technical field of plant cell engineering, in particular to a rapid propagation method for pinellia tissue culture. Background technique [0002] Pinellia (scientific name: Pinellia ternata) belongs to the order Araceae and is a medicinal plant, also known as "Dry Pinellia". my country's wild pinellia resources are mainly concentrated in Sichuan, Hubei, Anhui, Yunnan, and Guizhou provinces; the large amount of artificial cultivation is mainly in Gansu, Shanxi, Shandong, Guizhou, and Yunnan provinces. Pinellia is a shallow-rooted herb plant with a height of 15cm to 30cm. Most of them are single leaves. They grow from the top of the tuber and separate into three leaflets when they grow up. Plant buds with reproductive capacity can grow from the base of the petiole. The diameter of the underground tuber is generally 1cm to 2cm, nearly spherical, so it is often called "bulb" abroad. Pinellia is a monoecious plant, which is bo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘玲玲梁永琪段珍珍
Owner 甘肃源宜生物科技有限公司
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