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A technology of tissue culture and maple tree, applied in the field of plant tissue culture, can solve the problems of slow reproduction speed and low germination rate of seeds of maple tree, and achieve the effect of fast reproduction speed, neat and consistent offspring, and large reproduction coefficient.
Active Publication Date: 2016-11-09
海南热作两院种业科技有限责任公司
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[0005] The purpose of the present invention is to provide a method for tissue culture of Aurea japonica, which
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[0033] The present invention will be described in further detail below in conjunction with specific examples.
[0034] (1) Disinfection treatment
[0035] Take fresh mature seeds that are ripe, plump, and free from diseases and insect pests as explants, peel off the peel and clean them, soak them in an aqueous solution of about 2% washing powder or detergent for 5-10 minutes, and then rinse them with running water for about 1 hour. Soak and disinfect with 75% alcohol for 30-60 seconds on an ultra-clean workbench, then disinfect with 0.1% mercury liter for 10-12 minutes, then rinse with sterile water several times to remove the disinfectant for later use.
[0036] (2) Primary culture
[0037] The sterilized explants were cut open, and the intact embryos were removed and inoculated into different primary medium (MS medium with different amounts of 6-BA and NAA added, see Table 1 for details), at a culture temperature of 26±1°C. , cultured in a culture room with a light intensi...
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Abstract
The invention provides a daphniphyllum calycinum tissue culture method which comprises the following steps of disinfection treatment, primary culture, secondary culture, strong seedling culture, rooting culture and acclimatization and transplant. The method is used for daphniphyllum calycinum tissue culture, and has the advantages of being high in reproduction speed, large in reproduction coefficient, uniform in reproduced offspring and high in transplanting survival rate.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for culturing a maple tissue. Background technique [0002] Cow ear maple (Latin scientific name: Daphniphyllum calycinum Benth.) Alias: bell belly, pig belly fruit, pig belly wood, pig stomach, pig Luo wood, pig network wood, pig wood, Zhuluzi, goat dung, sheep dung , tiger ear, crow gall, big ear comb, Nanling tiger Nan, Nanling tiger skin Nan and Lingnan tiger skin Nan. It is an evergreen shrub or small tree plant of the genus Pachyphyllaceae, about 1-6 meters high, with gray-brown branchlets, sparse lenticels and leaf scars, about 3-5 mm in diameter. Produced in Guangxi, Guangdong, Fujian, Jiangxi and other provinces and regions; born in sparse forests or shrubs at an altitude of 250-700 meters, and also distributed in Vietnam and Japan. [0003] Squeezing oil from the seeds of the maple can be used to make soap or lubricating oil, and its ...
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