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Hybrid antimicrobial peptide mel-mytb and its application

A technology of hybrid antimicrobial peptides and bacterial strains, which can be used in applications, antibacterial drugs, peptides, etc., can solve the problems of strong toxicity of antibacterial peptides, gaps in antibacterial activity, and poor stability, and achieve good application prospects, enhanced antibacterial activity, and good antibacterial activity. The effect of thermal stability

Active Publication Date: 2019-05-14
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a certain gap in the antibacterial activity of antimicrobial peptides compared with antibiotics, and many antimicrobial peptides still have many problems such as strong toxicity, poor stability, and high cost.

Method used

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  • Hybrid antimicrobial peptide mel-mytb and its application
  • Hybrid antimicrobial peptide mel-mytb and its application
  • Hybrid antimicrobial peptide mel-mytb and its application

Examples

Experimental program
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Embodiment 1

[0026] 1 Materials and methods

[0027] 1.1 Materials

[0028] 1.1.1 Plasmids and strains:

[0029] 毕赤酵母X-33(His-Mut+)、表达载体pPICZα-A购于Invitrogen公司;Escherichiacoli ATCC25922、Staphylococcus aureus ATCC25923、Staphylococcus aureusATCC6538、Salmonella enterica ATCC13076、Salmonella typhimurium ATCC14028、Vibrio parahaemolyticus ATCC17802、Vibrio vulnificus ATCC27562、Bacillussubtilis ATCC6633E Save it for this lab.

[0030] 1.1.2 Main reagents

[0031] Restriction enzymes XholⅠ, XbalⅠ, SacⅠ, T4 ligase (purchased from Takara (Dalian) Co., Ltd.); Zeocin, Tricine, agarose gel recovery kit, plasmid extraction kit, DNA molecular mass marker, small molecular mass Protein Maker (purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd.); pMD18-T was preserved by our laboratory; other reagents were domestic analytically pure; polynucleotide and DNA sequencing was completed by BGI.

[0032] 1.2 Cloning of hybrid antimicrobial peptide Mel-MytB gene

[0033] The Mel-MytB hybri...

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Abstract

The invention discloses a hybrid antibacterial peptide Mel-MytB and an application thereof. A hybrid antibacterial peptide Mel-MytB gene is cloned into a pPICZalpha-A vector to construct a secretory recombinant yeast expression vector pPICZalpha-A-MEM, and a P. Pastoris receptor bacterium X-33 is transformed. The Mel-MytB hybrid antibacterial peptide having a molecular weight of about 3.0 kD is expressed under the control of an alcohol oxidase promoter. Antibacterial property researches show that the expressed product has broad-spectrum antibacterial activity, and has good antibacterial activity against most G- bacteria and G+ bacteria; and the hybrid antibacterial peptide has significantly better inhibition activity to common pathogenic bacteria than original antibacterial peptides. The hybrid antibacterial peptide Mel-MytB has thermal stability and acid stability. All above characteristics make the recombinant antibacterial peptide Mel-MytB have good application prospects in fields of disease prevention and control and animal feed additives.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a hybrid antibacterial peptide Mel-MytB and its application. Background technique [0002] Antimicrobial peptides are a class of polypeptide substances with broad-spectrum antibacterial activity. Some antimicrobial peptides can also activate the immune system, participate in cell signal transduction, regulate cell proliferation, and have anti-tumor, anti-virus, and anti-parasite functions. Antimicrobial peptides can destroy bacterial cell membranes, causing intracellular spillage to kill bacteria. The mechanism is different from that of antibiotics. Release, activate the immune system's recognition and presentation of antigens, and increase the differentiation, proliferation and response levels of immune cells. Because of its unique bactericidal mechanism and excellent physical and chemical properties, antimicrobial peptides have become a research hotspot for safe and gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00C12N15/11C12N1/19A61K38/16A61P31/04A23K20/147A23L33/18A23L3/3526
CPCA23K20/147A23L3/3526A23L33/18A23V2002/00A61K38/00A61P31/04C07K14/00A23V2200/10A23V2200/30A23V2250/55
Inventor 张宏刚李莉
Owner GUANGZHOU GLAM BIOTECH
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