Cell Affinity Detection Sample Device and Its Application in Cell Affinity Measurement

Abstract

The invention discloses a cell affinity detecting and sampling device and application thereof in determining cell affinity. The device comprises a sampler, a hard capillary, and a capillary joint, thehard capillary is of a tubular structure and the tubular structure is filled with cell fixing gel, one end of the hard capillary is provided with external threads, the capillary joint is of a tubularstructure, and one end is provided with internal threads and connected to one end of the threads of the hard capillary, a filter screen is also mounted in the capillary joint, the other end of the capillary joint is connected with a detecting device, the discharge end of the sampler is connected with the other end of the hard capillary, the sampler is used to input a working buffer solution and asolution to be tested, wherein the working buffer solution and the solution to be tested are injected into the hard capillary by the sampler to allow the working buffer solution and the solution to be tested to pass through cell fixing gel in the hard capillary, then be pushed out from the capillary joint and enter the detecting device. The device is low in cost, simple and convenient operation,and can carry out fast sample input to the detecting equipment.

Description

technical field [0001] The invention relates to the field of affinity detection of proteins and small molecular compounds, in particular to a cell affinity detection sample device and its application in cell affinity measurement. Background technique [0002] Drugs and proteins exert their therapeutic effects through interaction, which specifically refers to the binding of drugs to specific sites or regions of proteins through covalent bonds or non-covalent bonds such as hydrogen bonds, changing the spatial conformation of proteins Or organize the protein to undergo specific conformational changes to form a compound-protein complex (hereinafter referred to as the complex), thereby enhancing, weakening or losing a certain function of the protein. Therefore, the combination of drug and protein is the initial key process for its pharmacological activity. A "drug" here refers to a compound that has been shown to have a therapeutic effect. [0003] For the same target protein, ...

AI Technical Summary

Problems solved by technology

However, these techniques require the use of purified proteins for affinity detection, and cannot perform simple and effective qualitative comparisons of affinity, and the cost of equipment is high

[0007] On the other hand, for many proteins, especially cell surface receptor proteins, it is quite difficult to obtain proteins with natural activity and conformation

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