Method for inhibiting red tide algae, inhibiting liquid of red tide algae and preparation method of inhibiting liquid
A technology of red tide algae and inhibitory liquid, applied in botany equipment and methods, chemicals for biological control, animal repellent, etc., can solve problems such as high cost and secondary pollution
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[0062] Another embodiment of the present invention also provides a method for preparing the above-mentioned red tide algae inhibitory liquid, comprising the following steps:
[0063] Step 1: put sea iris in an oven and dry until the weight does not change, and grind the dried sea iris into sea iris powder with a grinder;
[0064] Step 2: adding solvent to the calamus calamus powder, stirring to dissolve the soluble substances in the solvent;
[0065] Step 3: filter to remove bacteria and particles, and obtain the extract of calamus calamus.
[0066] The solvent includes one or more of artificial seawater, ethanol and n-hexane;
[0067] When the solvent is artificial seawater, use a 0.22 μm cellulose acetate filter to remove particles and microorganisms;
[0068] When the solvent is ethanol or n-hexane, a 0.22 μm polyethersulfone filter membrane is used to filter out bacteria and particles.
[0069] When the solvent is ethanol or n-hexane, the obtained calamus extract is blo...
Embodiment 1
[0072] Example 1: Inhibitory effect of sea calamus water extract on four kinds of red tide algae
[0073] 1. The effect of the water extract of Acorus calamus on the cell density of four kinds of red tide algae
[0074] 1.1 Inhibitory effect of the water extract of Acorus calamus on Steinerella cone
[0075] The algae density of Steinerella coniformis in the water extract of Acorus calamus dry powder see figure 1 . Compared with the blank control group, 0.1g·L -1 The treatment group began to have an inhibitory effect on Steinerella cones on the fourth day, 0.1g·L -1 The density of algae in the treatment group was significantly lower than that of the blank control group from the fifth day to the eighth day (P-1 , 1g·L -1 , 2.5g·L -1 The density of algae in the treatment group was significantly lower than that in the control group (P-1 In the treatment group, the density of algae increased in the first three days, and the density of algae decreased from the fourth day, show...
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