Method of establishing mouse model of functional dyspepsia

A technology of indigestion and mouse models, which is applied in the field of medical animal model modeling, can solve problems such as irritability, incompletely consistent clinical patient disease process, and mouse tail injury, and achieve moderate stress intensity effects

Active Publication Date: 2018-11-16
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most animal models of functional dyspepsia adopt tail pinch method. The principle of pinch tail method is to use tail pinch to irritate mice to fight with other mice. In the long run, it will cause rats to be irritable and irritable, which will lead to symptoms of dyspepsia....

Method used

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  • Method of establishing mouse model of functional dyspepsia
  • Method of establishing mouse model of functional dyspepsia
  • Method of establishing mouse model of functional dyspepsia

Examples

Experimental program
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Embodiment 1

[0055] This embodiment provides a method for establishing a mouse model of functional dyspepsia, comprising the following steps:

[0056] S1, animal selection and pretreatment;

[0057] Twenty SPF grade Kunming mice were selected, each weighing about 20 g, and the mice were adaptively reared for 7 days before modeling. During rearing, water and feed were supplied adequately and the mice were allowed to drink freely.

[0058] After the adaptive feeding, the mice were divided into two groups, one group was the normal group and the other group was the modeling group, with 10 mice in each group.

[0059] S2, animal model establishment;

[0060] After the adaptive feeding, the modeling was started, and restraint, electric shock, and forced swimming were given in sequence every day. The three stimuli were as follows: After the mice were successfully restrained by restraint tubes, the mice were electroshocked for 5 minutes, and the mice were restrained continuously for 6 hours, and...

Embodiment 2-3

[0083] The modeling methods provided in Examples 2-3 are identical to the modeling methods provided in Example 1, except that the conditions are different.

Embodiment 2

[0085] The mice were reared adaptively for 5 days. The first electric shock was to shock the mouse's tail for 3 minutes, and the duration of restraint was 5.5 hours. The second electric shock was to shock the mouse's tail for 3 minutes.

[0086] Fatigue swimming is 45 minutes after the release of restraint, control mice to swim in water at 19°C, and the standard of fatigue is that the mouse's nose is submerged in the water for 2-4 seconds and cannot be lifted. Modeling for 28 days.

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Abstract

The invention relates to the field of medical animal model establishment, in particular to a method of establishing a mouse model of functional dyspepsia. The method comprises: carrying out constraint, electric shock, and fatigue swimming on a mouse successively each day and then carrying out continuous modeling of chronic unpredictable mild stresses randomly for 28 to 32 days. Because of the successive constraint, electric shock, and fatigue swimming on the mouse and then the random application of the chronic unpredictable mild stresses, the experimental animals have the functional dyspepsiasymptom similar to patients; and the bodies of the experimental animals are protected from being injured and no organic changing is caused because of the proper stress strength, so that characteristics of the functional dyspepsia are met.

Description

technical field [0001] The invention relates to the field of medical animal model modeling, in particular to a method for establishing a mouse model of functional dyspepsia. Background technique [0002] Functional dyspepsia, also known as non-ulcer dyspepsia, is a group of common clinical syndromes including persistent or recurrent epigastric discomfort, loss of appetite, early satiety, retrosternal pain discomfort, nausea, vomiting or other epigastric discomfort Symptoms that persist for at least 3 months without evidence of local or systemic organic disease. Due to the complex etiology, the pathogenesis of functional dyspepsia is still unclear, but it is generally believed to be related to abnormal gastrointestinal motility regulation mechanism, gastrointestinal hormone secretion disorder, abnormal gastric acid secretion, increased visceral sensitivity, brain-gut axis disorder, and bacterial infection. It is also related to psychological factors, among which abnormal gas...

Claims

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Application Information

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IPC IPC(8): A61N1/36
CPCA61N1/36
Inventor 王术玲龚彦溶
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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