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Method for establishing a mouse model of functional dyspepsia

A technology for indigestion and mouse models, applied in the field of medical animal model modeling, can solve problems such as irritability, infection, rat irritability, etc., and achieve the effect of moderate stress intensity

Active Publication Date: 2022-02-01
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most animal models of functional dyspepsia adopt tail pinch method. The principle of pinch tail method is to use tail pinch to irritate mice to fight with other mice. In the long run, it will cause rats to be irritable and irritable, which will lead to symptoms of dyspepsia.
In clinical practice, most patients with functional dyspepsia are depressed and depressed for a long time, showing a certain degree of depressive symptoms, rather than irritable and irritable. Exactly, and long-term tail pinching can damage the tail of the mouse, causing infection and other problems

Method used

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  • Method for establishing a mouse model of functional dyspepsia
  • Method for establishing a mouse model of functional dyspepsia
  • Method for establishing a mouse model of functional dyspepsia

Examples

Experimental program
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Effect test

Embodiment 1

[0055] This embodiment provides a method for establishing a mouse model of functional dyspepsia, comprising the following steps:

[0056] S1. Selection and pretreatment of animals;

[0057] 20 mice of SPF grade Kunming were selected, each weighing about 20 g, and the mice were adaptively fed for 7 days before modeling. During the feeding process, the water and feed were adequately supplied and the mice drank freely.

[0058] After the adaptive feeding, the mice were divided into two groups, one was the normal group and the other was the model group, with 10 mice in each group.

[0059] S2. Animal model establishment;

[0060] After the adaptive feeding was over, model building was started, and restraint, electric shock, and forced swimming were given in sequence every day. The three stimuli were operated as follows: After the mouse was successfully restrained with a restraint tube, the tail of the mouse was electroshocked for 5 minutes, and the restraint was continued for 6 ...

Embodiment 2-3

[0083] The modeling method provided in Examples 2-3 is in the same operation as the modeling method provided in Example 1, except that the conditions are different.

Embodiment 2

[0085] Adaptive feeding was carried out for 5 days. The first electric shock to the mice was to shock the tail of the mice for 3 minutes, and the time of continuous restraint was 5.5 hours. The second electric shock to the mice was to shock the tail of the mice for 3 minutes.

[0086] Fatigue swimming is to control the mice to swim in 19°C water 45 minutes after unshackled, and take the mouse's nose submerged in the water for 2-4 seconds and unable to lift it as the fatigue standard. 28 days for modeling.

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Abstract

The invention relates to the field of medical animal model modeling, in particular to a method for establishing a mouse model of functional dyspepsia. The method for establishing a mouse model of functional dyspepsia comprises the following steps: sequentially implementing restraint, electric shock and fatigue swimming to the mice every day, and then randomly applying chronic unpredictable mild stress to continue modeling for 28-32 days. Randomly applying chronic unpredictable mild stress to mice after restraint, electric shock and fatigue swimming sequentially makes experimental animals show symptoms of functional dyspepsia similar to clinical patients. Physical injuries, let alone organic changes, are consistent with the characteristics of functional dyspepsia.

Description

technical field [0001] The invention relates to the field of medical animal model modeling, in particular to a method for establishing a mouse model of functional dyspepsia. Background technique [0002] Functional dyspepsia, also known as non-ulcer dyspepsia, is a group of common clinical symptoms, including persistent or recurrent epigastric discomfort, loss of appetite, early satiety, retrosternal pain and discomfort, nausea, vomiting or other epigastric discomfort Symptoms that persist for at least 3 months without evidence of local or systemic organic disease. Due to the complex etiology, the pathogenesis of functional dyspepsia is not yet clear, but it is generally believed that it is related to abnormal gastrointestinal motility regulation mechanism, gastrointestinal hormone secretion disorder, abnormal gastric acid secretion, increased visceral sensitivity, brain-gut axis disorder, and bacterial infection It is related to psychological factors, among which abnormal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61N1/36
CPCA61N1/36
Inventor 王术玲龚彦溶
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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