Nucleic acid detection device based on microfluidic chip

Abstract

The invention relates to a nucleic acid detection device based on a microfluidic chip. The device comprises a chip chamber for accommodating a chip, a motor, two sample adding pumps, a radiator and acontroller, wherein the chip chamber comprises a chip chamber upper cover, a chip chamber middle ring and a chip chamber lower ring which are connected from top to bottom sequentially; the motor and the sample adding pumps are arranged on one side of the chip chamber, the sample adding pumps are driven by the motor to act to add a nucleic acid sample to the reaction chip; the radiator is arrangedon one side of the chip chamber and used for cooling the reaction chamber to control the temperature of the reaction chamber within a preset range; the controller completes a reaction test by controlling the rotation speed and temperature of the reaction chip as well as the motor; the chip comprises a chip upper layer, a chip lower layer and a chip middle layer which are arranged sequentially, andan FTA (filter paper) card is arranged in the chip middle layer. The device has high degree of automation, an operator can realize nucleic acid detection only by single sample addition, and control precision is high.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a nucleic acid detection device based on a microfluidic chip. Background technique [0002] In the prior art, with the rapid development of gene sequencing technology, gene sequencing has developed from traditional gene chip sequencing to current high-throughput gene sequencing. At present, gene sequencing systems and devices have made great progress in various aspects, but their development is still in its infancy, and various aspects still need continuous improvement and innovation. [0003] Gene sequencing equipment in the prior art is an instrument for measuring gene sequences, which includes a data processing device and a nucleic acid detection device. The data processing device is connected with the nucleic acid detection device for providing a user interface and controlling the operation of the nucleic acid detection device according to control instructions, and disp...

AI Technical Summary

Problems solved by technology

In this technical solution, the user can only know the working status of each component of the nucleic acid detection device by operating the data processing device, which is inconvenient to use

[0004] However, the nucleic acid detection devices in the prior art can only mix and detect a single type of nucleic acid, and especially cannot automatically control different reagents for different reaction temperatures and times, so that different nucleic acids can be processed according to the actual reaction time sequence and automatic response to temperature

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