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Chemical modification method for laccase and application of modified laccase

A technology of chemical modification and modification method, which is applied in the direction of biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problem that the modification effect of laccase is not obvious, so as to ensure the effect of chemical modification, improve the performance of paper making, Guarantee the effect of chemical modification effect

Active Publication Date: 2018-11-16
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and shortcomings of the insignificant modification effect of laccase in the prior art, and provide a chemical modification method of laccase, which protects the active site of laccase from being affected by adding laccase inhibitors , thus improving the chemical modification effect of laccase

Method used

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Embodiment 1

[0036] Example 1 ~ 11

[0037] A method for chemically modifying laccase, comprising the following steps: adding a laccase inhibitor to a potassium dihydrogen phosphate / dipotassium hydrogen phosphate (pH=7.70) buffer solution, then adding a laccase solution, and then sequentially adding potassium dihydrogen phosphate / Dipotassium hydrogen phosphate (pH = 7.70) buffer solution prepared carboxyl activator B and amino group-containing chemical modifier A, mix well, cross-linking reaction, after 60 min of reaction, add sodium acetate buffer solution (pH = 7.0) to terminate the reaction, Then the mixture was transferred to a dialysis bag filled with 0.1 mol / L sodium chloride solution for dialysis for 24 h to obtain the chemically modified laccase, and the volume was adjusted to 50 mL of chemically modified laccase.

[0038] The specific parameters are shown in Table 1 below.

[0039] Wherein the amino group-containing chemical modifier A of embodiments 1 to 8 is: formamidine acet...

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Abstract

The invention discloses a chemical modification method for laccase and application of modified laccase. The chemical modification method for laccase comprises the following steps: successively addinga laccase inhibitor, a carboxyl activator and an amino group-containing chemical modifier into an aqueous laccase solution, and carrying out dialysis on a reaction system after a reaction is completedso as to obtain chemically-modified laccase, wherein the amino group-containing chemical modifier is formamidine acetate or L-tryptophan methyl ester hydrochloride, and the carboxyl activator is 2-ethyl-5-phenylisoxazolium-3'-sulfonate. According to the chemical modification method for laccase, the laccase inhibitor is added in the process of a cross-linking reaction so as to protect the active center site of laccase from influence, so the active group, i.e., the carboxyl group, on a laccase chain is protected by forming a peptide bond through the cross-linking reaction of activated carboxylgroups and amino groups, which enables the chemical modification effect of the laccase to be effectively ensured. The chemically-modified laccase prepared in the invention can be widely applied to fiber modification for improvement of the paper making performance of fibers.

Description

technical field [0001] The invention belongs to the technical field of laccase modification, and more specifically relates to a laccase chemical modification method and application thereof. Background technique [0002] Emperor Bamboo Grass is a large plant with the characteristics of strong adaptability, strong tillering, fast growth, high yield, rich nutrition, high fiber content, and can be harvested for many years. It is a very valuable non-wood fiber pulping and papermaking Raw materials, but the strength of the paper produced is low, in order to improve its utilization level and use value, it is necessary to modify it. In terms of fiber modification, the biomodification of laccase mediator system is currently a research hotspot and difficulty. The biomodification of laccase mediator system has many advantages, such as mild reaction conditions, less fiber damage, and the use of environmentally friendly drugs, etc. , but because laccase has amino acid residues or termin...

Claims

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Application Information

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IPC IPC(8): C12N9/02D21C5/00
CPCC12N9/0061C12Y110/03002D21C5/005
Inventor 陈杨梅蒋雨吴启堂陈家进仇卓涛陈烁娜
Owner SOUTH CHINA AGRI UNIV
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