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A kind of laccase chemical modification method and its application

A modification method and chemical modification technology, applied in the direction of biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problem that the effect of laccase modification is not obvious, and achieve the goal of ensuring the effect of chemical modification and improving the performance of papermaking Effect

Active Publication Date: 2022-03-22
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and shortcomings of the insignificant modification effect of laccase in the prior art, and provide a chemical modification method of laccase, which protects the active site of laccase from being affected by adding laccase inhibitors , thus improving the chemical modification effect of laccase

Method used

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Examples

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Effect test

Embodiment 1

[0036] Example 1 ~ 11

[0037] A method for chemically modifying laccase, comprising the following steps: adding a laccase inhibitor to a potassium dihydrogen phosphate / dipotassium hydrogen phosphate (pH=7.70) buffer solution, then adding a laccase solution, and then sequentially adding potassium dihydrogen phosphate / Dipotassium hydrogen phosphate (pH = 7.70) buffer solution prepared carboxyl activator B and amino group-containing chemical modifier A, mix well, cross-linking reaction, after 60 min of reaction, add sodium acetate buffer solution (pH = 7.0) to terminate the reaction, Then the mixture was transferred to a dialysis bag filled with 0.1 mol / L sodium chloride solution for dialysis for 24 h to obtain the chemically modified laccase, and the volume was adjusted to 50 mL of chemically modified laccase.

[0038] The specific parameters are shown in Table 1 below.

[0039] Wherein the amino group-containing chemical modifier A of embodiments 1 to 8 is: formamidine acet...

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Abstract

The invention discloses a laccase chemical modification method and application thereof. The method for chemically modifying laccase comprises the following steps: sequentially adding a laccase inhibitor, a carboxyl activator, and an amino group-containing chemical modifier to the laccase aqueous solution, and after the reaction is complete, dialyzing the reaction system to obtain a chemically modified laccase, the amino group-containing chemically modified The agent is formamidine acetate or L-tryptophan methyl ester hydrochloride, and the carboxyl activator is 2-ethyl-5-phenylisoxazole-3'-sulfonate. Laccase chemical modification method In the process of cross-linking reaction, laccase inhibitors are added to protect the active center of laccase from being affected, so that the cross-linking reaction between the activated carboxyl group and the amino group forms a peptide bond, so that the active group on the laccase chain The group carboxyl group is protected, effectively ensuring the chemical modification effect of laccase. The chemically modified laccase prepared by the invention can be widely used in fiber modification to improve the papermaking performance of the fiber.

Description

technical field [0001] The invention belongs to the technical field of laccase modification, and more specifically relates to a laccase chemical modification method and application thereof. Background technique [0002] Emperor Bamboo Grass is a large plant with the characteristics of strong adaptability, strong tillering, fast growth, high yield, rich nutrition, high fiber content, and can be harvested for many years. It is a very valuable non-wood fiber pulping and papermaking Raw materials, but the strength of the paper produced is low, in order to improve its utilization level and use value, it is necessary to modify it. In terms of fiber modification, the biomodification of laccase mediator system is currently a research hotspot and difficulty. The biomodification of laccase mediator system has many advantages, such as mild reaction conditions, less fiber damage, and the use of environmentally friendly drugs, etc. , but because laccase has amino acid residues or termin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02D21C5/00
CPCC12N9/0061C12Y110/03002D21C5/005
Inventor 陈杨梅蒋雨吴启堂陈家进仇卓涛陈烁娜
Owner SOUTH CHINA AGRI UNIV
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