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Detection kit and application thereof in assessing schizophrenia genetic risk

A detection kit and technology for schizophrenia, applied in the direction of microbial determination/examination, biochemical equipment and methods, etc., can solve the problems of delayed diagnosis and treatment, difficult treatment, lack of tools to provide objective support for clinical diagnosis, etc. The effect of reducing burden, wide product coverage, and ensuring accuracy

Inactive Publication Date: 2018-11-16
广州海思医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Schizophrenia mostly occurs in young and middle-aged people, and the early symptoms are relatively hidden. Due to the difficulty of early identification, it often leads to delays in diagnosis and treatment and difficulties in treatment
Moreover, the course of the disease is often protracted, accounting for more than half of the inpatients in the psychiatric department, and about half of the patients end up with mental disabilities, which brings a serious burden to the society, patients and their families
At present, the early identification of high-risk groups for schizophrenia in the world mainly relies on symptoms to judge, such as whether the patient has mild positive symptoms and short-term and limited intermittent psychotic symptoms, and it is assisted by genetic risk analysis. Domestic research on schizophrenia Diagnosis is mainly based on ICD-10, CCMD-3 and DSM-5 standards, and the subjectivity of clinicians has a great influence on the diagnosis results
Clinically, there is an extreme lack of tools that can provide objective support for physicians' clinical diagnosis

Method used

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  • Detection kit and application thereof in assessing schizophrenia genetic risk
  • Detection kit and application thereof in assessing schizophrenia genetic risk
  • Detection kit and application thereof in assessing schizophrenia genetic risk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: A method for assessing the genetic risk of schizophrenia using the detection kit

[0056] A method for evaluating the genetic risk of schizophrenia using the detection kit, which specifically includes the following steps:

[0057] (1) Take a human peripheral anticoagulant blood sample, first use a commercial nucleic acid extraction kit to extract human genomic DNA samples, and test the extracted DNA, requiring A260 / A280 between 1.6-2.0, concentration ≥ 20ng / μL . The concentration of DNA and the ratio of A260 / A280 in the present embodiment are as follows:

[0058] A260 / A280

C (ng / μL)

1.70

87

[0059] (2) Take 20 μL of capture reaction solution, add 1.2 μL of DNA extracted in step (1) and 3.8 μL of purified water;

[0060] Wherein the composition of capture reaction solution is as follows:

[0061] components

Volume (μL)

5×PCR buffer

5

dNTP Mix(10mM)

0.25

DNA Polymerase (10U / μL)

0.6

...

Embodiment 2

[0084] (1) using the DNA sample extracted by (1) step in Example 1;

[0085] (2) Take 20 μL of capture reaction solution, add 1.2 μL of DNA extracted in (1) and 3.8 μL of purified water

[0086]

[0087]

[0088] All the other conditions are the same as in Step 2 of Example 1.

[0089] After the PCR amplification was completed, the PCR product was detected by electrophoresis on a 2% agarose gel, and the loading volume of the PCR product was 5 μL. (See figure 2 )

[0090] (3) Product purification

[0091] The purification steps are the same as Step 3 of Example 1. Use Qseq100 to detect the fragment size after product purification, where image 3 is the fragment size after purification of the product obtained according to condition 1; Figure 4 is the fragment size after purification of the product obtained according to condition 2; Figure 5 is the fragment size after purification of the product obtained according to condition 3; Image 6 is the fragment size aft...

Embodiment 3

[0093] Take a clinical anticoagulant blood sample, operate according to the steps in Example 1, and sequence the samples that pass the quality inspection on a high-throughput sequencer. The sequencing depth of each site is as follows Figure 7 shown. It can be seen from the figure that the samples processed by this kit were sequenced on a high-throughput sequencer, and all 123 sites were successfully sequenced, and the uniformity of the sequencing depth of each site was good.

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Abstract

The invention discloses a detection kit and application thereof in assessing the schizophrenia genetic risk, and belongs to the field of gene detection. The prior art is short of detection kits of methods used for assessing the schizophrenia genetic risk. The detection kit contains 123 susceptibility gene sites which are related to the schizophrenia illness risk and screened out through a GWAS study or obtained from a pathophysiology pathway according to pathogenesis. According to a detection result of patient susceptibility genes, a reference can be provided for diagnosis of a clinical doctor, illness risk assessment can be conducted on normal people who have familial-hereditary disease histories, by means of the detection kit and the application thereof in assessing the schizophrenia genetic risk, a void of a schizophrenia early risk assessment product on the national market is filled, screening can be conducted on people efficiently in a large scale, and effects and load of schizophrenia are greatly reduced.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a detection kit and its application for evaluating the genetic risk of schizophrenia. Background technique [0002] Mental illness refers to the various degrees of obstacles in mental activities such as cognition, emotion, will and behavior of individuals under the influence of biological and environmental factors. Common mental illnesses include: schizophrenia, bipolar disorder, depression, autism, Alzheimer's disease, etc. Schizophrenia is the most serious mental illness, which is mainly characterized by the division of basic personality, thinking, emotion and behavior, and the incoordination between mental activities and the environment, manifested as hallucinations, delusions, depression, pessimism, etc. [0003] In 1991, Gottesman published a study on twins and an analysis of families, showing that relatives of schizophrenic patients have a higher prevalence than t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6869
CPCC12Q1/6883C12Q1/6869C12Q2535/122C12Q2525/191C12Q2563/143C12Q2563/149
Inventor 周煌凯钟诗龙陈秋红刘勋姚啟聪陆嫚云邓美英王芳
Owner 广州海思医疗科技有限公司