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Quick detection method for SNP loci of peripheral blood leucocytes

A detection method and white blood cell technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve good application prospects, simple operation process, and short operation time

Inactive Publication Date: 2018-11-20
济南广音医疗科技有限公司
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Problems solved by technology

[0004] In order to overcome the drawbacks of existing multiple detection methods that restrict the detection of SNP sites, the present invention provides a method that does not require a professional laboratory, and the detection personnel do not need PCR certificates, low detection cost, simple detection process, and short detection time. A rapid detection method of SNP sites in peripheral blood leukocytes with short and accurate detection results

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  • Quick detection method for SNP loci of peripheral blood leucocytes

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[0015] figure 1 As shown in , a rapid detection method for SNP sites in peripheral blood leukocytes, using ammonium chloride solution, nanoporous spheres, and hexaoligonucleotides as the supplies used for detection, ammonium chloride solution, nanoporous spheres, hexaoligonucleotides The composition ratios of the nucleotide components are: 0.74% ammonium chloride solution, 0.3% nanoporous balls, and 10UM hexaoligonucleotide.

[0016] figure 1 As shown in , a rapid detection method for SNP sites in peripheral blood leukocytes, the detection of SNP sites in peripheral blood leukocytes is completed through the following steps. The first step is to take the patient's EDTA anticoagulated blood sample; The amount is 2ml. The second step is to separate and enrich white blood cells; the process of separating and enriching white blood cells is as follows, put the ammonium chloride solution and 2 ml of the patient's DTA anticoagulant blood sample into the test tube and mix well, after...

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Abstract

The invention relates to a quick detection method for SNP loci of peripheral blood leucocytes. The quick detection method is characterized by detecting the SNP loci of peripheral blood leucocytes through the following steps by taking an ammonium chloride solution, a nanoporous ball, hexaoligonucleotide as articles for detection: S1, taking an EDTA anticoagulant sample of a patient; S2, separatingand enriching leucocytes; S3, incising and segmenting the enriched leucocytes; S4, melting double-stranded DNA of the segmented leucocytes to generate single-stranded DNA and combining the single-stranded DNA with fluorescently-labeled hexaoligonucleotide to generate a hybrid fragment by means of a base complementary principle of DNA; and S5, feeding the hybrid fragment into the nanoporous ball, carrying out detection by a fluorescent detector, establishing a group of intact gene fluorescence labeling maps, carrying out calculation by a computer, and finally, obtaining the needed SNP locus genomic sequence. Without a special lab and a PCR work license, the quick detection method is low in detection cost, simple in process, short in time and accurate in result.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a rapid detection method for SNP sites of peripheral blood leukocytes. Background technique [0002] SNP, the English full name is single nucleotide polymorphism, single nucleotide polymorphism, mainly refers to the DNA sequence polymorphism caused by the variation of a single nucleotide at the genome level, it is the most genetically mutated gene in humans The common one, accounting for more than 90% of all known polymorphisms. SNPs exist widely in the human genome, with an average of 1 in every 500-1000 base pairs, and it is estimated that the total number can reach 3 million or more. Due to the large number of SNP sites in the human body, the differences in the SNP sites of each person form different genotypes, which determine people's different risks of diseases and different responses to drugs, and give people the opportunity to discover various diseases, among...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6834
CPCC12Q1/6834C12Q2563/155
Inventor 崔铮
Owner 济南广音医疗科技有限公司
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