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Online milk somatic cell electrical detection device and method based on micro-fluidic technique

A technology of milk somatic cells and microfluidic technology, which is applied in the field of on-line electrical detection devices for milk somatic cells, can solve problems such as the difficulty in realizing the quantitative detection of milk somatic cells, the inability to obtain accurate values ​​of somatic cells, and the complicated operation process, etc., to achieve Realize on-line rapid measurement, on-line quantitative detection, simple operation process

Inactive Publication Date: 2018-11-20
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The direct detection method requires expensive measuring equipment such as microscopes and high-speed cameras, and the detection cost is high
The indirect detection method mainly uses test paper to obtain the somatic cell count of milk indirectly by measuring the pH value of milk. This method can only estimate the approximate range of somatic cell count in milk, but cannot obtain the precise value of somatic cell count in milk.
Therefore, the currently commonly used online detection technology for the number of milk somatic cells has two main disadvantages: 1) The operation process is complicated and the detection cost is high: before the detection, the milk somatic cells need to be fluorescently labeled, and during the detection process, microscopes, high-speed Cameras and other optical equipment collect images, and obtain milk somatic cell counts through online image processing
2) Low detection accuracy: In order to reduce the detection cost, methods such as measuring the pH value of milk are used to indirectly obtain milk somatic cell counts, and it is difficult to achieve quantitative detection of milk somatic cell counts

Method used

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specific Embodiment approach 1

[0027] Specific implementation mode one: the following combination figure 1 Describe this embodiment, the milk somatic cell online electrical detection device based on microfluidic technology described in this embodiment is composed of an air supply source 1, a pressure reducing valve 2, a first pressure sensor 3, a first liquid container 4, a first section Flow valve 5, second pressure sensor 6, second liquid container 7, second throttle valve 8, T-shaped micro flow channel 9, tiny detection electrode 10, capacitance sensor 11 and microprocessor 12 constitute. in:

[0028] The gas inlet of the pressure reducing valve 2 is communicated with the outlet of the gas supply source 1, and the gas outlet of the pressure reducing valve 2 is communicated with the gas inlets of the first liquid container 4 and the second liquid container 7;

[0029] The liquid outlet of the first liquid container 4 is connected with the liquid inlet of the first throttle valve 5, and the liquid outlet ...

specific Embodiment approach 2

[0038] Specific implementation mode two: the following combination figure 2 This embodiment will be described, and this embodiment will further describe the first specific embodiment. The electrical detection method needs to arrange multiple pairs of tiny detection electrodes on both sides of the T-shaped micro-channel, and measure the capacitance signal output by the tiny detection electrodes in real time to obtain the number of milk droplets. The tiny detection electrodes are symmetrically distributed on both sides of the T-shaped micro-channel, and T The spacing between adjacent electrodes on the same side of the T-shaped micro-channel is consistent, and there is a certain gap between the end of the detection electrode and the T-shaped micro-channel, which is non-contact measurement.

[0039]In this embodiment, the width of the tiny detection electrode is 200 µm, the width w of the T-shaped micro-channel is 100 µm, the gap c between the end of the detection electrode and t...

specific Embodiment approach 3

[0041] Specific implementation mode three: the following combination image 3 Describe this embodiment, and this embodiment will further explain Embodiment 1. In order to realize the on-line quantitative detection of milk somatic cells, the electrical detection method collects the capacitance signal output by the detection electrode in real time, and performs signal processing by the microprocessor. The signal processing process It includes: the capacitance sensor measures the capacitance at both ends of the tiny detection electrode, the capacitance value is read by the data acquisition card, and the capacitance value is transmitted to the microprocessor in real time, and the milk somatic cell count is obtained after signal processing.

[0042] The measurement range of the capacitance sensor is: 0-4 pF, the measurement accuracy is 4 fF, and the maximum capacitance acquisition frequency is 100Hz.

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Abstract

The invention discloses an online milk somatic cell electrical detection device and method based on a micro-fluidic technique. The device comprises an air supply source, a reducing valve, a pressure sensor, a liquid container, a throttling valve, a T-shaped micro-channel, micro detection electrodes, a capacitive sensor and a microprocessor, wherein the reducing valve is communicated with the air supply source and the liquid container; the liquid container is connected with the throttling valve; the throttling valve is communicated with the T-shaped micro-channel; the micro detection electrodesare arranged on the upper and lower sides of the T-shaped micro-channel in pairs; the micro detection electrodes are connected with the capacitive sensor; the capacitive sensor is connected with themicroprocessor; the pressure sensor is connected with the microprocessor; the microprocessor is connected with the throttling valve. According to the device disclosed by the invention, the micro-fluidic chip, the micro detection electrodes and the micro-fluidic flow regulation device are integrated into a whole, the detection accuracy of the milk somatic cell count can be improved, the detection cost is reduced, and online quantitative determination of the milk somatic cell count is realized.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, and relates to an on-line electrical detection device and method for milk somatic cells. Background technique [0002] Microfluidic technology has important applications in many disciplines such as chemistry, materials, biology, medicine and food. In particular, in food science, since milk somatic cell count is an important indicator reflecting milk quality and the health of dairy cows, how to quickly and accurately detect animal somatic cell counts in milk is of great importance for ensuring milk quality and monitoring the health of dairy cows. significance. At present, the detection methods of milk somatic cell count are mainly divided into direct detection method and indirect detection method. The most typical direct detection method is the fluorescence microscope counting method, which requires fluorescent labeling of the cells, and then completes the online detection of milk somatic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/22G01N33/04
CPCG01N27/22G01N27/221G01N33/04
Inventor 曾文李松晶符海贾伟亮
Owner HARBIN INST OF TECH
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